Publicación: Cladóceros en el desarrollo del sistema digestivo de larvas de blanquillo Sorubim cuspicaudus
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The Trans-Andean Shovelnose catfish (Sorubim cuspicaudus) is an important species in the Colombian fishery with potential for cultivation due to the quality of its meat, high commercial value and good adaptation to captivity; their larvae have a reduced vitelline reserve and slow development of the digestive tract, which causes high mortality rates, thus, the availability of a nutritious diet at the beginning of exogenous feeding is essential. Within zooplankton, cladocerans are a form of living food suitable for their nutritional and enzymatic value, size, color and mobility. This research evaluated the influence of two cladocerans on the growth and development of the digestive system of tile larvae. In a completely randomized design, tile larvae with three days post hatching, at the beginning of their endoexotrophic feeding, were distributed in density of 25 larvae. L-1 in 10 L glass aquariums; for five days they were fed with four treatments: T1: Artemia nauplii (control), T2: Macrothrix sp., T3: Moina sp. and T4: Macrothrix sp. + Moina sp. (50:50), at a feeding density of 10 individuals. mL-1 , for a total of 100.000 prey per aquarium, offered three times a day. The performance parameters mortality (Ma), survival (S), resistance to stress (Re), weight gain (GP) and length (GL) and instantaneous growth rate (TCE) were evaluated. Histological and histochemical techniques using PAS, Alcian blue, Masson's Trichomic and Toluidine staining were performed to determine the morphology and development of the larval digestive system at the beginning and end of the experimental period. Daily, the temperature (27.70 ± 0.33 °C), dissolved oxygen (5.84±0.19 mg. L-1 ) and pH (7.93±0.06 units) were recorded. The cladocerans Macrothrix sp. and Moina sp. presented an average length of 453.7 and 698.5 µm, and an average width of 289.5 and 412.6 µm, respectively. The lowest percentage of mortality was presented with T4 (2.53±0.87 %), the highest percentage of survival with T2 (86.53±2.74 %) and the highest percentage of Re with T3 (93.33±3.33 %); the highest GP (4.7±0.24 mg), GL (4.05±0.06 mm) and TCE (37.14±0.85 %) was recorded with T1. Except for the resistance to stress of the larvae, all the treatments showed a significant difference (p