Publicación:
Caracterización molecular de la microbiota bacteriana cervical y del virus de papiloma humano (VPH) en muestras de mujeres provenientes de dos regiones de Colombia

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dc.audience
dc.contributor.advisorSoto De León, Sara Cecilia
dc.contributor.authorArteaga Pautt, Heiser
dc.contributor.juryAlvarez Aldana, Adalucy
dc.contributor.jurySierra Verbel, Álvaro
dc.contributor.juryArroyo Salgado, Bárbara
dc.date.accessioned2025-06-27T20:16:26Z
dc.date.available2025-06-27T20:16:26Z
dc.date.issued0025-06-27
dc.description.abstractEl cáncer cervical (CC) es una de las principales preocupaciones de salud pública en países en desarrollo, ocupando el cuarto lugar entre los tipos más comunes de cáncer que afectan a mujeres. El factor de riesgo predominante en el desarrollo de CC es la infección por el Virus del Papiloma Humano de alto riesgo (VPH-AR). Esta infección se caracteriza por variables intrínsecas del virus como el genotipo infectante, la carga viral y la presencia de múltiples genotipos. Además, factores individuales, ambientales y conductuales como el consumo de alcohol, tabaquismo y el uso de anticonceptivos hormonales también influyen significativamente. Recientemente, la microbiota cervical ha ganado atención debido a su potencial influencia en la susceptibilidad y progresión del VPH. En la región Caribe de Colombia, hay una falta de estudios sobre la frecuencia específica de los tipos de VPH-AR. Este estudio se enfocó en determinar la frecuencia de VPH-AR y su asociación con variables sociodemográficas en mujeres que asisten a controles de citología cervical en la IPS Liga Cordobesa Contra el Cáncer. También se investigó la composición del microbiota cervical en mujeres infectadas con VPH-AR, diferenciando entre infecciones únicas y múltiples. Participaron voluntariamente 450 mujeres, quienes, tras firmar un consentimiento informado, completaron una encuesta que recopiló datos sociodemográficos, clínicos y de comportamiento sexual. Se les realizaron pruebas de citología cervical y análisis molecular mediante PCR. De las muestras positivas, se tipificaron los VPH con el kit de Optiplex HPV Genotyping. Para el análisis de la microbiota cervical, se seleccionaron 60 muestras con cantidad óptima de ADN y se procesaron con la técnica de Ilumina.spa
dc.description.abstractCervical cancer (CC) is a major public health concern in developing countries, ranking fourth among the most common cancers affecting women. The primary risk factor for developing CC is infection with high-risk types of Human Papillomavirus (HPV-AR). This infection is characterized by intrinsic viral variables such as the infecting genotype, viral load, and the presence of multiple genotypes. Additionally, individual, environmental, and behavioral factors like alcohol consumption, smoking, and the use of hormonal contraceptives significantly influence the risk. Recently, cervical microbiota has attracted attention for its potential role in influencing HPV susceptibility and progression. In the Colombian Caribbean region, there is a notable lack of studies on the specific frequency of high-risk HPV types (HPV-AR). This study aimed to determine the type-specific frequency of HPV-AR and its association with sociodemographic variables in women attending cervical cytology screening at the IPS Liga Cordobesa Contra el Cáncer. The study also explored the cervical microbiota composition in women infected with HPV-AR, distinguishing between single and multiple infections. The study involved 450 voluntary participants who, after signing informed consent forms, completed a survey collecting sociodemographic, clinical, and sexual behavior information. Each woman underwent cervical cytology and molecular analysis through Polymerase Chain Reaction (PCR). Positive samples were genotyped using the Optiplex HPV Genotyping kit. For cervical microbiota analysis, 60 samples with optimal DNA quantities were selected and processed using Illumina sequencing techniques.eng
dc.description.degreelevelDoctorado
dc.description.degreenameDoctor(a) en Microbiología y Salud Tropical
dc.description.modalityTrabajos de Investigación y/o Extensión
dc.description.tableofcontentsABREVIATURAS.. 8
dc.description.tableofcontentsRESUMEN 9
dc.description.tableofcontentsABSTRACT 12
dc.description.tableofcontentsESTRUCTURA DEL TRABAJO 15
dc.description.tableofcontentsINTRODUCCIÓN 17
dc.description.tableofcontentsOBJETIVOS 29
dc.description.tableofcontentsCAPITULO I 30
dc.description.tableofcontentsCAPITULO 2 39
dc.description.tableofcontentsCAPITULO 3 25
dc.description.tableofcontentsCAPITULO 4 62
dc.description.tableofcontentsCONCLUSIONES FINALES 76
dc.description.tableofcontentsRECOMENDACIONES 78
dc.description.tableofcontentsPERSPECTIVAS ........79
dc.description.tableofcontentsCONSIDERACIONES ÉTICAS 80
dc.description.tableofcontentsREFERENCIAS BIBLIOGRÁFICAS 81
dc.description.tableofcontentsANEXOS 93
dc.description.tableofcontentsANEXO 1: CONSENTIMIENTO INFORMADO 94
dc.description.tableofcontentsANEXO 2: FICHA EPIDEMIOLÓGICA 99
dc.description.tableofcontentsANEXO 3: ACTA DE COMITÉ ÉTICA 100
dc.description.tableofcontentsANEXO 4: ARTÍCULO CIENTÍFICO PUBLICADO 101
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad de Córdoba
dc.identifier.reponameRepositorio Institucional Unicórdoba
dc.identifier.repourlhttps://repositorio.unicordoba.edu.co
dc.identifier.urihttps://repositorio.unicordoba.edu.co/handle/ucordoba/9223
dc.language.isospa
dc.publisher.facultyFacultad de Medicina Veterinaria y Zootecnia
dc.publisher.placeMontería, Córdoba, Colombia
dc.publisher.programDoctorado en Microbiología y Salud Tropical
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dc.rightsCopyright Universidad de Córdoba, 2025
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordsWorld Health Organization
dc.subject.keywordsSexually transmitted infection
dc.subject.keywordsPelvic inflammatory disease HPV
dc.subject.keywordsHuman papillomavirus DNA
dc.subject.keywordsDeoxyribonucleic acid RNA
dc.subject.keywordsRibonucleic acid
dc.subject.keywordsCervical cancer
dc.subject.keywordsHigh-risk human papillomavirus
dc.subject.keywordsPolymerase chain reaction
dc.subject.keywordsLow-grade squamous intraepithelial lesion
dc.subject.keywordsHigh-grade squamous intraepithelial lesion
dc.subject.keywordsBase pairs, Upstream regulatory region
dc.subject.keywordsOrigin of replication
dc.subject.keywordsLong control region
dc.subject.keywordsLate promoter
dc.subject.keywordsEarly promoter
dc.subject.keywordsCervical intraepithelial neoplasia
dc.subject.keywordsAtypical squamous cells of undetermined significance
dc.subject.keywordsSquamous cell carcinoma
dc.subject.keywordsAdenocarcinoma
dc.subject.keywordsHigh-risk human papillomavirus
dc.subject.keywordsLow-risk human papillomavirus
dc.subject.proposalOrganización Mundial de la Salud
dc.subject.proposalInfección de transmisión sexual
dc.subject.proposalEnfermedad Pélvica Inflamatoria VPH
dc.subject.proposalVirus de Papiloma Humano ADN
dc.subject.proposalÁcido desoxirribonucleico ARN
dc.subject.proposalÁcido Ribonucleico
dc.subject.proposalCancer cervical
dc.subject.proposalVirus de Papiloma humano de alto riesgo
dc.subject.proposalReacción en cadena de la polimerasa
dc.subject.proposalLesión escamosa intraepitelial de bajo grado
dc.subject.proposalLesión escamosa intraepitelial de alto grado
dc.subject.proposalPares de bases
dc.subject.proposalRegión reguladora corriente arriba
dc.subject.proposalOrigen de replicación
dc.subject.proposalRegión Larga de control
dc.subject.proposalPromotor región tardía
dc.subject.proposalPromotor región temprana
dc.subject.proposalNeoplasia intraepitelial cervical intraepitelial
dc.subject.proposalCélulas escamosas atípicas de significado indeterminado
dc.subject.proposalCarcinoma de células escamosas
dc.subject.proposalAdenocarcinoma
dc.subject.proposalVirus de Papiloma Humano de alto riesgo
dc.subject.proposalVirus de Papiloma Humano de bajo riesgo
dc.titleCaracterización molecular de la microbiota bacteriana cervical y del virus de papiloma humano (VPH) en muestras de mujeres provenientes de dos regiones de Colombia
dc.typeTrabajo de grado - Doctorado
dc.type.coarhttp://purl.org/coar/resource_type/c_db06
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/doctoralThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TD
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
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