Publicación:
Evaluación del potencial antifúngico del isoespintanol extraído de Oxandra xylopioides Diels (Annonaceae), contra aislamientos intrahospitalarios de Candida tropicalis

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dc.contributor.advisorSantafé Patiño, Gilmar
dc.contributor.authorContreras Martínez, Orfa Inés
dc.contributor.jurySáez Vega, Alex
dc.contributor.juryPajaro Castro, Nerlis
dc.contributor.juryPérez Hernández, Edwin
dc.date.accessioned2024-01-29T21:32:41Z
dc.date.available2024-01-29T21:32:41Z
dc.date.issued2024-01-26
dc.description.abstractActualmente, la incidencia de Infecciones Asociadas a la Atención en Salud (IAAS) representa un grave problema por el aumento, no solo de los índices de morbilidad y mortalidad, sino también los costos en los servicios de salud a nivel global. Los hongos son uno de los principales agentes etiológicos de estas infecciones; infectan a un gran número de personas cada año aumentando las tasas de morbi-mortalidad especialmente en personas inmunocomprometidas. Recientemente se ha reportado la eficacia de compuestos de origen vegetal con potencial antifúngico como una alternativa a ser empleada. Por esto, el objetivo de esta investigación fue evaluar el potencial antimicrobiano del isoespintanol (ISO), tomando como modelo la levadura patógena Candida tropicalis, estimar su capacidad para inhibir biopelículas y hacer una exploración a los mecanismos de acción antifúngica de este compuesto. Los resultados revelaron inhibición del crecimiento fúngico e inhibición de biopalículas, con efectos superiores al efecto causado por la anfotericina B (AFB). Además, se evidenció el efecto del ISO sobre varias dianas moleculares como mecanismo de acción antifúngica. En cuanto al efecto del ISO, sobre el transcriptoma de C. tropicalis, el análisis de expresión génica diferencial permitió una visión integral de los cambios fisiológicos, estructurales y metabólicos de C. tropicalis que ocurren en presencia de ISO. Esta investigación resalta que la actividad antifúngica de ISO es un proceso complejo que involucra múltiples objetivos de acción.spa
dc.description.degreelevelDoctorado
dc.description.degreenameDoctor(a) en Microbiología y Salud Tropical
dc.description.modalityTrabajos de Investigación y/o Extensión
dc.description.tableofcontentsRESUMEN 3spa
dc.description.tableofcontentsABSTRACT 5eng
dc.description.tableofcontentsCAPITULO 1. INTRODUCCIÓN 7spa
dc.description.tableofcontents1.1. Problema y Justificación 7spa
dc.description.tableofcontents1.2. OBJETIVOS 10spa
dc.description.tableofcontents1.2.1. Objetivo general 10spa
dc.description.tableofcontents1.2.2. Objetivos específicos 10spa
dc.description.tableofcontentsCAPITULO 2. MARCO TEÓRICO 11spa
dc.description.tableofcontents2.1. Bases teóricas 11spa
dc.description.tableofcontents2.1.1. Infecciones Asociadas a la Atención en Salud (IAAS)spa
dc.description.tableofcontents2.1.2. Infecciones por Candida spp.spa
dc.description.tableofcontents2.1.3. Candida tropicalisspa
dc.description.tableofcontents2.1.4. Factores de virulencia en Candida tropicalisspa
dc.description.tableofcontents2.1.5. Mecanismos de resistenciaspa
dc.description.tableofcontents2.1.6. La familia Annonaceaespa
dc.description.tableofcontents2.1.7. El género Oxandraspa
dc.description.tableofcontents2.1.8. Oxandra xylopioides Dielsspa
dc.description.tableofcontents2.1.9. Metabolitos secundariosspa
dc.description.tableofcontents2.1.10. Isoespintanol 17spa
dc.description.tableofcontents2.2. Antecedentes 19spa
dc.description.tableofcontentsCAPITULO 3. METODOLOGÍA 21spa
dc.description.tableofcontents3.1. Aislamiento, Purificación, e Identificación del Isoespintanol 21spa
dc.description.tableofcontents3.2. Ensayos de citotoxicidad del isoespintanol 21spa
dc.description.tableofcontents3.2.1. Cultivo celular 22spa
dc.description.tableofcontents3.2.2. Ensayo de MTT 22spa
dc.description.tableofcontents3.2.3. Ensayo de viabilidad con CV 22spa
dc.description.tableofcontents3.3. Sensibilidad de bacterias y levaduras del género Candida frente al isoespintanol. 23spa
dc.description.tableofcontents3.3.1. Sensibilidad de bacterias frente al ISO 24spa
dc.description.tableofcontents3.3.2. Sensibilidad de levaduras del género Candida frente al ISO 25spa
dc.description.tableofcontents3.4. Filogenómica de Candida tropicalis 26spa
dc.description.tableofcontents3.4.1. Extracción de ADN genómico 26spa
dc.description.tableofcontents3.4.2. Secuenciación genómica de C. tropicalis WGS (Whole genome shotgun) 27spa
dc.description.tableofcontents3.5. Ensayos de sensibilidad antifúngica de C. tropicalis 27spa
dc.description.tableofcontents3.5.1. Ensayo de reducción de MTT 27spa
dc.description.tableofcontents3.5.2. Curva de inhibición del crecimiento fúngico 28spa
dc.description.tableofcontents3.6. Exploración de mecanismos de acción antifúngico del isoespintanol contra C. tropicalis mediante su acción sobre diferentes dianas 28spa
dc.description.tableofcontents3.6.1. Efecto del ISO sobre la membrana celular fúngica 28spa
dc.description.tableofcontents3.6.1.1. Ensayos con citometría de flujo empleando Ioduro de propidio (IP) 28spa
dc.description.tableofcontents3.6.1.2. Pérdida de material intracelular a través de la membrana celular 29spa
dc.description.tableofcontents3.6.1.3. Medida de pH extracelular 29spa
dc.description.tableofcontents3.6.1.4. Ensayos LIVE/DEAD 30spa
dc.description.tableofcontents3.6.1.5. Tinción con azul de Evans 30spa
dc.description.tableofcontents3.6.1.6. Determinación del contenido de ergosterol 30spa
dc.description.tableofcontents3.6.2. Efecto del ISO sobre la morfología e integridad de las células 31spa
dc.description.tableofcontents3.6.3. Efecto del ISO sobre la producción de especies reactivas de oxígeno intracelular (EROi) 32spa
dc.description.tableofcontents3.6.4. Efecto del ISO sobre el potencial de membrana mitocondrial (ΔΨm) 32spa
dc.description.tableofcontents3.6.5. Efecto del ISO sobre biopelículas fúngicas 33spa
dc.description.tableofcontents3.6.6. Efecto del ISO sobre la integridad de la pared celular 34spa
dc.description.tableofcontents3.7. Efecto del ISO sobre el transcriptoma de C. tropicalis 35spa
dc.description.tableofcontents3.7.1. Secuenciación de ARN y datos de recuentos de lectura. 35spa
dc.description.tableofcontents3.7.2. Análisis bioinformático 35spa
dc.description.tableofcontentsCAPITULO 4. RESULTADOS Y DISCUSIÓN 37spa
dc.description.tableofcontentsCAPITULO 5. CONCLUSIONES 38spa
dc.description.tableofcontentsReferencias 39spa
dc.description.tableofcontentsAnexos 50spa
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad de Córdoba
dc.identifier.reponameRepositorio Universidad de Córdoba
dc.identifier.repourlhttps://repositorio.unicordoba.edu.co/
dc.identifier.urihttps://repositorio.unicordoba.edu.co/handle/ucordoba/8132
dc.language.isospa
dc.publisherUniversidad de Córdoba
dc.publisher.facultyFacultad de Medicina Veterinaria y Zootecnia
dc.publisher.placeMontería, Córdoba, Colombia
dc.publisher.programDoctorado en Microbiología y Salud Tropical
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dc.rightsCopyright Universidad de Córdoba, 2024
dc.rights.accessrightsinfo:eu-repo/semantics/openAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordsOxandra xylopioides
dc.subject.keywordsIsoespintanol
dc.subject.keywordsAntifungal potential
dc.subject.proposalOxandra xylopioides
dc.subject.proposalIsoespintanol
dc.subject.proposalPotencial antifúngico
dc.titleEvaluación del potencial antifúngico del isoespintanol extraído de Oxandra xylopioides Diels (Annonaceae), contra aislamientos intrahospitalarios de Candida tropicalisspa
dc.typeTrabajo de grado - Doctorado
dc.type.coarhttp://purl.org/coar/resource_type/c_db06
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/doctoralThesis
dc.type.redcolhttp://purl.org/redcol/resource_type/TD
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
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