Publicación:
Identificación molecular de ADN Anaplasma spp y Dirofilaria Immitis en murciélagos del departamento de Córdoba

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dc.audience
dc.contributor.advisorMartínez Bravo, Caty Milena
dc.contributor.authorBertel Pacheco, Valeria
dc.contributor.juryContreras Cogollo, Verónica
dc.contributor.juryCalderón Rangel, Alfonso
dc.date.accessioned2024-07-10T22:52:22Z
dc.date.available2025-07-10
dc.date.available2024-07-10T22:52:22Z
dc.date.issued2024-07-10
dc.description.abstractDebido a características biológicas y evolutivas, como la longevidad, supervivencia, grandes densidades de población, comportamiento de descanso y la capacidad de volar; los murciélagos son considerados como potenciales reservorios de patógenos zoonóticos en todo el mundo. Estudios recientes identificaron a los murciélagos como hospederos potenciales del filo de las proteobacterias y de nematodos filariales. El objetivo de este estudio fue determinar por medio de pruebas moleculares la presencia de ADN de Anaplasma spp. y Dirofilaria immitis en murciélagos del departamento de Córdoba. En este estudio se analizaron muestras de tejidos de murciélagos mediante detección del gen ARNr 16s de la familia Anaplasmataceae y el gen Citocromo oxidasa para Dirofilaria immitis. El 2,42% (5/206) muestras de bazo fueron positivas para el gen ARN 16s y el 26,34% (54/205) muestras de tejido de corazón para el gen (COX). Murciélagos del género Artibeus fueron los huéspedes con mayor presencia de infección por los distintos hemotrópicos. Se evidenció una coinfección en dos individuos de las especies Artibeus phaeotis y Phyllostomus hastatus, registrados en los municipios de Montelíbano y Moñitos, respectivamente. Nuestro estudio presenta la primera detección de D. immitis en murciélagos en el departamento de Córdoba. Factores como los cambios en el uso del suelo (agricultura, deforestación, urbanización, ganadería, etc.), se encontraron relacionados con la circulación de hemotrópicos con potencial zoonótico en murciélagos del departamento de Córdoba. Estos resultados fortalecen el esquema de salud unificada mediante la prevención y detección de enfermedades zoonóticas integrando la salud animal y el bienestar ecosistémico que sin duda repercute en el bienestar y salud humana. Palabras clave: PCR convencional, secuenciación, Anaplasma spp, Dirofilaria immitis, murciélagos.spa
dc.description.abstractDue to biological and evolutionary characteristics such as longevity, survival, high population densities, resting behavior, and the ability to fly, bats are considered potential reservoirs of zoonotic pathogens worldwide. Recent studies have identified bats as potential hosts of the Proteobacteria phylum and filarial nematodes. The aim of this study was to determine, through molecular testing, the presence of DNA of Anaplasma spp. and Dirofilaria immitis in bats from the department of Córdoba. In this study, bat tissue samples were analyzed by detecting the 16s rRNA gene of the Anaplasmataceae family and the Cytochrome oxidase gene for Dirofilaria immitis. 2.42% (5/206) spleen samples were positive for the 16s rRNA gene and 26.34% (54/205) heart tissue samples for the (COX) gene. Bats of the genus Artibeus were the hosts with the highest presence of infection by the different hemotropics. A co-infection was evidenced in two individuals of the species Artibeus phaeotis and Phyllostomus hastatus, recorded in the municipalities of Montelíbano and Moñitos, respectively. Our study presents the first detection of D. immitis in bats in the department of Córdoba. Factors such as changes in land use (agriculture, deforestation, urbanization, livestock, etc.), were found to be related to the circulation of hemotropics with zoonotic potential in bats in the department of Córdoba. These results strengthen the unified health scheme through the prevention and detection of zoonotic diseases by integrating animal health and ecosystem well-being, which undoubtedly impacts human health and well-being. Keywords: Conventional PCR, sequencing, Anaplasma spp, Dirofilaria immitis, bats.eng
dc.description.degreelevelPregrado
dc.description.degreenameBiólogo(a)
dc.description.modalityTrabajos de Investigación y/o Extensión
dc.description.tableofcontents1. INTRODUCCIÓN...9
dc.description.tableofcontents2. OBJETIVOS...12
dc.description.tableofcontents2.1. OBJETIVO GENERAL...12
dc.description.tableofcontents2.2. OBJETIVOS ESPECÍFICOS...12
dc.description.tableofcontents3. MARCO TEÓRICO Y ANTECEDENTES...13
dc.description.tableofcontents3.1. Anaplasma spp. 13
dc.description.tableofcontents3.1.1. Estado del arte...13
dc.description.tableofcontents3.2. Dirofilaria spp...15
dc.description.tableofcontents3.2.1. Estado del arte...16
dc.description.tableofcontents3.3. Zoonosis como problema de salud pública...17
dc.description.tableofcontents3.4. Antecedentes de los murciélagos como potenciales reservorios zoonóticos...19
dc.description.tableofcontents4. MATERIALES Y MÉTODOS...22
dc.description.tableofcontents4.1. Área de estudio...22
dc.description.tableofcontents4.2. Muestreo en campo y toma de muestras...23
dc.description.tableofcontents4.3. Protocolos de laboratorio...23
dc.description.tableofcontents4.3.1. Extracción de ADN...23
dc.description.tableofcontents4.3.2. Estandarización de protocolo para PCR...23
dc.description.tableofcontents4.3.3. Revelación de productos por electroforesis...24
dc.description.tableofcontents4.4. Secuenciación...25
dc.description.tableofcontents4.5. Elaboración árbol filogenético...25
dc.description.tableofcontents4.6. Georreferenciación...25
dc.description.tableofcontents5. RESULTADOS Y DISCUSIÓN...26
dc.description.tableofcontents5.1. Resultados para el cumplimiento del objetivo específico 1...26
dc.description.tableofcontents5.1.1 Extracción de ácidos nucleicos...26
dc.description.tableofcontents5.1.2. Ensayo de PCR por multiplexación para la detección molecular de ADN hemotrópicos...27
dc.description.tableofcontents5.1.3. Estandarización de protocolo para la detección de ADN Anaplasma spp...28
dc.description.tableofcontents5.1.4. Estandarización de protocolo para la detección de ADN Dirofilaria immitis...29
dc.description.tableofcontents5.2. Resultados para el cumplimiento del objetivo específico 2...30
dc.description.tableofcontents5.2.1. Detección de Anaplasma spp...30
dc.description.tableofcontents5.2.2. Detección de Dirofilaria immitis...33
dc.description.tableofcontents5.2.3. Análisis filogenéticos...36
dc.description.tableofcontents5.3. Resultados para el cumplimiento del objetivo 3...37
dc.description.tableofcontents6. CONCLUSIONES...41
dc.description.tableofcontents7. RECOMENDACIONES...42
dc.description.tableofcontents8. BIBLIOGRAFÍA...43
dc.description.tableofcontents9. ANEXOS...53
dc.format.mimetypeapplication/pdf
dc.identifier.instnameUniversidad de Córdoba
dc.identifier.reponameRepositorio Universidad de Córdoba
dc.identifier.repourlhttps://repositorio.unicordoba.edu.co
dc.identifier.urihttps://repositorio.unicordoba.edu.co/handle/ucordoba/8370
dc.language.isospa
dc.publisherUniversidad de Córdoba
dc.publisher.facultyFacultad de Ciencias Básicas
dc.publisher.placeMontería, Córdoba, Colombia
dc.publisher.programBiología
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dc.rightsCopyright Universidad de Córdoba, 2024
dc.rights.accessrightsinfo:eu-repo/semantics/embargoedAccess
dc.rights.coarhttp://purl.org/coar/access_right/c_f1cf
dc.rights.licenseAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.keywordsConventional PCR
dc.subject.keywordsSequencing
dc.subject.keywordsAnaplasma spp
dc.subject.keywordsDirofilaria immitis
dc.subject.keywordsBats
dc.subject.proposalPCR convencional
dc.subject.proposalSecuenciación
dc.subject.proposalAnaplasma spp
dc.subject.proposalDirofilaria immitis
dc.subject.proposalMurciélagos
dc.titleIdentificación molecular de ADN Anaplasma spp y Dirofilaria Immitis en murciélagos del departamento de Córdobaspa
dc.typeTrabajo de grado - Pregrado
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1f
dc.type.coarversionhttp://purl.org/coar/version/c_ab4af688f83e57aa
dc.type.contentText
dc.type.driverinfo:eu-repo/semantics/bachelorThesis
dc.type.versioninfo:eu-repo/semantics/acceptedVersion
dspace.entity.typePublication
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