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Evaluación del potencial antibacteriano y antibiopelícula de extractos etanólicos de Duguetia vallicola (Annonaceae) contra aislados clínicos de Pseudomonas aeruginosa

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dc.contributor.advisorContreras Martínez, Orfa Inésspa
dc.contributor.advisorAngulo Ortíz, Alberto Antoniospa
dc.contributor.authorSierra Quiroz, Daniela
dc.date.accessioned2023-07-12T16:19:35Z
dc.date.available2023-07-12T16:19:35Z
dc.date.issued2024-07-05
dc.description.abstractPseudomonas aeruginosa es un patógeno oportunista causante de infecciones nosocomiales a nivel mundial, la resistencia que expresa ante los antibióticos complica el tratamiento terapéutico, por lo que es apremiante la búsqueda de nuevas estrategias para su control. El efecto de diversos extractos y compuestos de origen vegetal con potencial antibacteriano son hoy día una de las alternativas más. Por ello, el objetivo de este estudio fue evaluar el potencial antibacteriano, antibiopelícula y la acción de los extractos de MDR y CTZ sobre la membrana celular bacteriana. Los ensayos de sensibilidad se realizaron bajo el método de microdilución en caldo MH, la erradicación de biopelículas maduras se hizo con cultivos puros 24 horas de crecimiento, tratados y teñidos con cristal violeta y posteriormente leídos a 590nm. El daño a la membrana se evaluó en células tratadas previamente con los extractos y respectivos controles, a través de mediciones en el gradiente de pH extracelular, fuga de material intracelular (260/280nm), y microscopia de fluorescencia empleando NA y BE. Nuestros resultados mostraron inhibición del crecimiento bacteriano (más del 50%), con la concentración más baja empleada (62.5μg/mL) de ambos extractos. Además, reportamos la capacidad de los extractos de CTZ y MDR de erradicar las biopelículas maduras (entre el 48.4% y 93.7%) y causar daño en la membrana celular Los extractos etanólicos de CTZ y MDR D. vallicola son promisorios en la búsqueda de compuestos novedosos con potencial antibacteriano.spa
dc.description.degreelevelPregradospa
dc.description.degreenameBiólogo(a)spa
dc.description.modalityArtículospa
dc.description.tableofcontentsABSTRACT………………………………………………………………………………………1spa
dc.description.tableofcontentsINTRODUCCION……………………………………………………………………………………2spa
dc.description.tableofcontentsMATERIALES Y MÉTODOS…………………………………………………………………………3spa
dc.description.tableofcontentsRESULTADOS…………………………………………………………………………………………………6spa
dc.description.tableofcontentsDISCUSIÓN………………………………………………………………………………………………………16spa
dc.description.tableofcontentsCONCLUSIÓN………………………………………………………………………………………………………20spa
dc.description.tableofcontentsRECOMENDACIONES………………………………………………………………………………………………20spa
dc.description.tableofcontentsREFERENCIAS………………………………………………………………………………………………………………21spa
dc.description.tableofcontentsANEXOS…………………………………………………………………………………………………………………………27spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.urihttps://repositorio.unicordoba.edu.co/handle/ucordoba/7415
dc.language.isospaspa
dc.publisher.facultyFacultad de Ciencias Básicasspa
dc.publisher.placeMontería, Córdoba, Colombiaspa
dc.publisher.programBiologíaspa
dc.rightsCopyright Universidad de Córdoba, 2023spa
dc.rights.accessrightsinfo:eu-repo/semantics/embargoedAccessspa
dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.subject.keywordsDuguetia vallicolaeng
dc.subject.keywordsAntibacterial activityeng
dc.subject.keywordsAntibiofilmeng
dc.subject.keywordsEthanolic extractseng
dc.subject.keywordsPseudomonas aeruginosaeng
dc.subject.proposalDuguetia vallicolaspa
dc.subject.proposalActividad antibacterianaspa
dc.subject.proposalAntibiopelículaspa
dc.subject.proposalExtractos etanólicosspa
dc.subject.proposalPseudomonas aeruginosaspa
dc.titleEvaluación del potencial antibacteriano y antibiopelícula de extractos etanólicos de Duguetia vallicola (Annonaceae) contra aislados clínicos de Pseudomonas aeruginosaspa
dc.typeTrabajo de grado - Pregradospa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/articlespa
dc.type.redcolhttps://purl.org/redcol/resource_type/ART
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
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