Publicación: Evaluación de la actividad antifúngica del flavonoide diosmina frente a aislados clínicos de Candida spp
dc.contributor.advisor | Contreras Martínez, Orfa Inés | |
dc.contributor.advisor | Angulo Ortiz, Alberto Antonio | |
dc.contributor.author | Rivera Castillo, Norella Esther | |
dc.contributor.jury | Villegas Gonzalez, Jazmith Paola | |
dc.contributor.jury | Peñata Taborda, Ana Marcela | |
dc.date.accessioned | 2025-07-18T18:48:04Z | |
dc.date.available | 2025-07-18T18:48:04Z | |
dc.date.issued | 2025-07-18 | |
dc.description.abstract | Las infecciones fúngicas, especialmente la candidiasis, representan un desafío creciente para la salud pública debido a su alta mortalidad, resistencia antifúngica y costos hospitalarios elevados (CDC, 2024). En este contexto, la búsqueda de nuevas alternativas terapéuticas es crucial, y los flavonoides emergen como una opción prometedora. El objetivo de este estudio fue evaluar el potencial antifúngico del flavonoide diosmina (DIO) frente a aislamientos clínicos de Candida spp., mediante la determinación de su concentración mínima inhibitoria (CMI), su capacidad para inhibir biopelículas fúngicas, y su efecto sobre la integridad de la membrana en Candida spp. DIO mostró actividad inhibidora frente a los siete aislamientos clínicos evaluados, con valores de CMI₉₀ entre 1150 y 2251 µg/mL. Asimismo, se observó una inhibición marcada en la formación de biopelículas, y una reducción significativa en biopelículas maduras, con un efecto particularmente notable frente a C. auris. Por otro lado, los ensayos de liberación de material intracelular evidenciaron daño en la permeabilidad de la membrana, luego de ser expuesta a DIO lo que sugiere un mecanismo de acción asociado a la pérdida de integridad celular. Adicionalmente, se exploró el efecto combinado de DIO con antifúngicos comerciales, revelando interacciones sinérgicas relevantes: en 4 de los 7 aislamientos se observó sinergismo con fluconazol, y en 5 con anfotericina B, destacando nuevamente el efecto sobre C. auris. En conjunto, estos hallazgos respaldan el potencial de DIO como agente antifúngico multifuncional, con capacidad de interferir en la viabilidad, estructura y resistencia de Candida spp., y aportan evidencia valiosa para el desarrollo futuro de terapias basadas en compuestos naturales. | spa |
dc.description.abstract | Fungal infections, particularly candidiasis, represent a growing public health challenge due to their high mortality rates, antifungal resistance, and elevated hospital costs (CDC, 2024). In this context, the search for new therapeutic alternatives is crucial, and flavonoids have emerged as a promising option. The aim of this study was to evaluate the antifungal potential of the flavonoid diosmin (DIO) against clinical isolates of Candida spp., by determining its minimum inhibitory concentration (MIC), its ability to inhibit fungal biofilms, and its effect on membrane integrity in Candida spp. DIO exhibited inhibitory activity against all seven clinical isolates tested, with MIC₉₀ values ranging from 1150 to 2251 µg/mL. A marked inhibition of biofilm formation was observed, as well as a significant reduction in mature biofilms, with a particularly notable effect against C. auris. Moreover, assays measuring the release of intracellular material revealed damage to membrane permeability following exposure to DIO, suggesting a mechanism of action associated with the loss of cellular integrity. Additionally, the combined effect of DIO with commercial antifungals was explored, revealing relevant synergistic interactions: synergy with fluconazole was observed in 4 out of 7 isolates, and with amphotericin B in 5 out of 7, again highlighting the effect against C. auris. Taken together, these findings support the potential of DIO as a multifunctional antifungal agent capable of interfering with the viability, structure, and resistance of Candida spp., and provide valuable evidence for the future development of therapies based on natural compounds. | eng |
dc.description.degreelevel | Pregrado | |
dc.description.degreename | Biólogo(a) | |
dc.description.modality | Trabajos de Investigación y/o Extensión | |
dc.format.mimetype | application/pdf | |
dc.identifier.instname | Universidad de Córdoba | |
dc.identifier.reponame | Repositorio Universidad de Córdoba | |
dc.identifier.repourl | https://repositorio.unicordoba.edu.co/ | |
dc.identifier.uri | https://repositorio.unicordoba.edu.co/handle/ucordoba/9411 | |
dc.language.iso | spa | |
dc.publisher | Universidad de Córdoba | |
dc.publisher.faculty | Facultad de Ciencias Básicas | |
dc.publisher.place | Montería, Córdoba, Colombia | |
dc.publisher.program | Biología | |
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dc.relation.references | 71. Vitális, E., Nagy, F., Tóth, Z., Forgács, L., Bozo, A., Kardos, G., Mayoros, L., & Kovács, R. (2020). La producción de biopelículas de Candida se asocia con una mayor mortalidad en pacientes con candidemia. Micosis, 63, 352–360 | |
dc.relation.references | 72. Welsh, R., Bentz, M., Shams, A., Houston, H., Lyons, A., Rose, L., & Litvintseva, A. (2017). Survival, Persistence, and Isolation of the Emerging Multidrug-Resistant Pathogenic Yeast Candida auris on a Plastic Health Care Surface. Journal of Clinical Microbiology, 55, 2996 - 3005. https://doi.org/10.1128/JCM.00921-17 | |
dc.relation.references | 73. Yarmolinsky, L., Budovsky, A. , Yarmolinsky, L. , Khalfin, B. , Glukhman, V. y Ben-Shabat, S. ( 2019 ). "Efecto de los fitoquímicos bioactivos de phlomis viscosa poiret sobre la cicatrización de heridas" . Plantas , 8 ( 12 ), 609 . | |
dc.relation.references | 74. Yarmolinsky, L., Nakonechny, F., Budovsky, A., Zeigerman, H., Khalfin, B., Sharon, E., ... & Nisnevitch, M. (2023). Antimicrobial and antiviral compounds of Phlomis viscosa Poiret. Biomedicines, 11(2), 441. | |
dc.relation.references | 75. Zheng, Y., Zhang, R., Shi, W., Li, L., Liu, H., Chen, Z., & Wu, L. (2020). Metabolism and pharmacological activities of the natural health-benefiting compound diosmin. Food & function, 11(10), 8472-8492. | |
dc.rights | Copyright Universidad de Córdoba, 2025 | |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | |
dc.rights.license | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.keywords | Diosmina | eng |
dc.subject.keywords | Antifungal | eng |
dc.subject.keywords | Biofilm | eng |
dc.subject.keywords | Candida spp | eng |
dc.subject.proposal | Diosmina | spa |
dc.subject.proposal | Antifungico | spa |
dc.subject.proposal | Biopelículas | spa |
dc.subject.proposal | Candida spp | spa |
dc.title | Evaluación de la actividad antifúngica del flavonoide diosmina frente a aislados clínicos de Candida spp | spa |
dc.type | Trabajo de grado - Pregrado | |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | |
dc.type.content | Text | |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | |
dspace.entity.type | Publication |
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