Examinando por Materia "Diversidad genética"
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Publicación Embargo Caracterización molecular de guayaba (Psidium guajava) en San Bernardo del Viento Córdoba utilizando marcadores moleculares tipo microsatélites(2024-07-05) León Genes, Camila María; Pardo Pérez, EnrriqueThe objective was to evaluate the variability and genetic structure of P. guajava using microsatellite markers in five subpopulations of San Bernardo del Viento in the department of Córdoba. Forty accessions of P. guajava, DNA was extracted with the chemical method CTAB 2X and the DNA concentration was quantified in a NanoDrop; seven microsatellite markers were amplified by PCR. Then analyzed in 8% polyacrylamide gels in a vertical electrophoresis chamber, the population genetic parameters were calculated, the analysis of molecular variance (AMOVA), the Polymorphic Information Content (PIC) and an analysis of the population genetic structure was performed by Bayesian inference. An average of 3.4 alleles per locus was recorded, a high level of homozygotes was found and a genetic variability of Fst= 0.584 was obtained; the population was not found in Hardy Weinberg equilibrium, this was mainly associated to inbreeding (F= 0.973), the AMOVA test reflected greater variations among individuals with 78%; according to Bayesian inference, all individuals evaluated would present similar genetic groups and the genetic distance allows us to determine that the subpopulations present a reduced gene flow. The population of P. guajava may present a genetic risk due to low heterozygosity indexes and monomorphisms, so it is suggested to implement plans for the preservation of materials in germplasm banksPublicación Acceso abierto Caracterización morfoagronómica y nutricional de 30 genotipos de fríjol caupí [Vigna unguiculata (L.) WALP.](2021-06-01) Fuentes Espitia, William José; Araméndiz Tatis, HermesCowpea beans [Vigna unguiculata (L.) WALP.], Is one of the oldest sources of human food and, given the high cost of animal proteins, is the most economical viable protein source, therefore it represents one of the the most important vegetable crops in the world and the main food crops in Africa, Latin America and India; In the Caribbean region of Colombia it is of great social, economic and nutritional importance, since it is part of the diet of all its inhabitants, who consume it in different presentations, this makes it an alternative that partially mitigates problems of malnutrition in vulnerable sectors of various departments in this part of the country. The University of Córdoba has in custody a collection of germplasm of this species made up of 55 entries between Creole and foreign materials, without morphoagronomic characterization; Morphoagronomic characterization studies are important for the conservation of phytogenetic resources, also for the knowledge of phenotypic variation, the relationship between genotypes and the identification of parents with characteristics of interest to obtain new genetic materials, in programs of improvement of a species . In the present study, 30 cowpea bean accessions were evaluated, based on 36 morphoagronomic characters, taking as reference the descriptors of the species generated by the IBPGR, multivariate analysis techniques called principal component analysis and cluster analysis were used in the identification of characters. Discriminators for the quantification of the genetic diversity of the evaluated germplasm, and identification of materials with interesting traits, especially vigor, grain size, grain color and iron, zinc and protein contents. The results demonstrated the existence of genetic variability and the contribution of some characters to this, the most contrasting accessions for traits of agronomic and nutritional interest, are suggested as progenitors of future hybridizations to take advantage of heterosis, this in order to improve yield and quality. Nutritional status of existing genotypes.Publicación Sólo datos Caracterización morfológica, faneróptica y de genes dominantes de la gallina criolla Subespecie nudicollis en la región Sabanas del departamento de Sucre-Colombia(Universidad de Córdoba, 2021-11-30) Montes-Vergara, Donicer; Hernández-Herrera, Darwin; Carrillo-González, DiegoPublicación Acceso abierto Diversidad genética en poblaciones humanas de Sincelejo, Sucre-Colombia, determinada mediante polimorfismos de inserciones Alu.(2021-07-21) Conde vega, Eder Enrique; Pardo Pérez, EnriqueThe objective of this research was to determine the genetic diversity of the human population of Sincelejo, Sucre-Colombia through polymorphism of Alu insertions. Materials and Methods. The samples were obtained from 49 unrelated people in five localities of this population (Bogotá, Caribe, 20 de enero, Villa country, Cortijo), to which the DNA extraction protocol described in the Promega Wizard® Genomic DNA Purification Kit Technical Manual is applied, and were amplified by a series of polymerase chain reactions (PCR) using a series of Alu markers (ACE, A25, APO, D1, PV92, TRA25). Results. All the Alu markers evaluated in this research were polymorphic, the allelic frequencies presented an average of 0.305 with a maximum value of 0.551 for the TRA25 locus and a minimum value of 0.064 for the APO locus, a moderate heterozygosity observed in the population was detected, where the values presented a fluctuation of a minimum value of 0.125 for the APO locus and a maximum value of 0.471 for the PV92 locus. Marker D1 presents an absence of Hardy-Weinberg equilibrium for two subpopulations of Sincelejo (Villa Country and Caribe). Conclusion. The results obtained in this research allow us to conclude that the population of Sincelejo, Sucre-Colombia has low genetic diversity due to the fact that inbreeding processes have occurred throughout the history of this population.Publicación Acceso abierto Diversidad genética en poblaciones humanas mediante polimorfismos de inserción Alu en el municipio de Moñitos- Córdoba(2020-11-13) Álvarez Villegas, Lizky Paola; Pardo Pérez, EnriqueAlu insertion elements are DNA sequences that are produced by a single mutational event and their ancestral status is known, it is estimated that there are more than a million copies of these elements, that is, they occupy approximately 11% of the human genome. The objective of the present investigation was to determine the genetic diversity of the human population of the municipality of Moñitos-Córdoba, through the use of Alu insertion polymorphisms in 50 unrelated individuals from five selected populations in the municipality. The study was carried out from saliva samples, from which the DNA was extracted, later the DNA amplification was carried out by PCR and the amplified ones were observed in agarose gels, finally through statistical analysis the following were determined: allelic frequencies, Observed heterozygosities (Ho), Expected heterozygosities (He), Fixation index, Hardy-Weinberg equilibrium and Dendograms. All Alu markers were polymorphic, with frequencies ranging between 0.100 (A25) and 0.944 (HS3.23), the average values obtained for Ho and He were respectively 0.398 and 0.384, most of the markers were found in equilibrium of Hardy-Weinberg, except for the ACE and TRA25 markers, the genetic distance between the different populations studied, showed Miramar and Flores de Manga as the most related and Santa Lucia as the most distant among the populations evaluated. In conclusion, Moñitos population presented a high polymorphic level and low values of observed and expected heterozygosity, which indicates a low rate of genetic diversity.Publicación Sólo datos Diversidad genética en seis poblaciones de tilapia roja, usando microsatelites como marcadores genéticos(Universidad de Córdoba, 2011-05-10) Briñez R, Boris; Caraballo O, Xenia; Salazar V, MarcelaPublicación Embargo Diversidad genética y estructura poblacional de Psidium guajava en Valencia Córdoba-Colombia utilizando marcadores moleculares tipo STR’S(2024-07-13) Palacio Durango, Camila; Pardo Pérez, EnriqueObjetivo. El objetivo de esta investigación fue evaluar la variabilidad y estructura genética de P. guajava L. en la población de Valencia del departamento de Córdoba, utilizando marcadores moleculares tipo microsatélites. Materiales y Métodos. Se analizaron 31 accesiones de Psidium guajava L., para la extracción de ADN se utilizó el método Mini-prep con modificaciones a partir de hojas jóvenes deshidratadas. Se evaluaron siete marcadores microsatélites; los productos de amplificación se analizaron mediante electroforesis vertical en geles de poliacrilamida al 8%. Resultados. Se encontraron un total de 26 alelos con un promedio de 3.7 alelos por locus. Las frecuencias alélicas oscilaron entre 0,063 y 0,929. La Heterocigosidad observada (Ho) y esperada (He) presentó valores promedio de 0.097 y 0.261 respectivamente indicando baja diversidad genética en las accesiones. El índice de fijación (F) presentó una media de 0,607 mostrando un exceso de homocigotos. La prueba de Hardy-Weinberg mostró que dos marcadores presentaron desequilibrios. Conclusión. Los 31 germoplasmas de Psidium guajava L. presentaron una baja diversidad genética reflejada en un alto número de homocigotos y niveles bajos de heterocigosidad. Además, las poblaciones, aunque presentan una diferenciación genética entre sí, tienden a agruparse al proceder de la misma región, lo que sugiere que las accesiones estudiadas corresponden a individuos emparentados.Publicación Acceso abierto Diversidad y estructura genética de la guayaba dulce (Psidium guajava l.) evaluada mediante marcadores microsatélites en tres municipios de Córdoba-Colombia(2022-07-25) Begambre Hernández, Mauricio; Pardo Pérez, EnriqueSweet guava (Psidium guajava L.) is a fruit tree of the Myrthaceae family with a wide distribution in tropical and subtropical areas, it is of great commercial interest due to its fruits with a pleasant flavor and high nutritional value. Due to its economic importance and its autochthonous condition in the department of Córdoba-Colombia, three populations of P. guajava corresponding to the municipalities of Sahagún, Tierralta and Cereté were characterized by microsatellite-type molecular markers (SSR). Of the twelve markers evaluated, seven were polymorphic and presented a total of 44 alleles distributed in three populations. The Polymorphic Information Content (PIC) was variable in the territories, finding the markers mPgCIR07 and mPgCIR20 as highly informative. The heterozygosity observed was 0.179, 0.081 and 0.555 for Sahagún, Tierralta and Cereté, respectively. The coefficient of differentiation between populations has an average of FST = 0.271 and a percentage of interpopulation variation of 7%, these results were reflected in the Bayesian clustering with DeltaK = 3 as the most probable number of genetic groups and in the differential distribution of the three populations in the principal coordinate analysis (PCoA). Cereté was characterized by a high genetic diversity as a result of the high family agricultural activity and productivity of this species in the soils of the present municipality, while the guava populations of the municipalities of Tierralta and Sahagún presented high inbreeding and a high interpopulation genetic differentiation was found, probably attributed to little interest in its production.Publicación Embargo Evaluación de la diversidad y variabilidad genética de guayaba (Psidium guajava) mediante marcadores microsatélites en el municipio de Montería, departamento de Córdoba-Colombia(Universidad de Córdoba, 2023-11-08) Ramos Rivero, Elizabeth; Pardo, Enrique; Causil Vargas, Luis AlfonsoLa guayaba (Psidium guajava L.) es un árbol frutal perteneciente a la familia Myrtaceae, cultivada en países tropicales y subtropicales. Sus frutos poseen excelentes propiedades nutricionales y medicinales importantes para la salud humana. Esta especie está ampliamente distribuida en el departamento de Córdoba, a pesar de esto, son escasos los estudios que se han llevado a cabo para conocer su diversidad genética. Por este motivo, el objetivo de esta investigación fue evaluar la diversidad y variabilidad genética de la guayaba (Psidium guajava) usando marcadores microsatélites en el municipio de Montería, departamento de Córdoba-Colombia. Se evaluaron 24 accesiones de P. guajava. Para la extracción de ADN se empleó el protocolo de extracción CTAB 2x con modificaciones. El ADN extraído se amplificó mediante la técnica PCR utilizando siete marcadores microsatélites; los amplicones se analizaron en gel de poliacrilamida al 8%. Se halló un total de 46 alelos con un promedio de 6,5 alelos por locus. Se identificó un total de 28 alelos privados en las poblaciones, los cuales posiblemente se han fijado por distintas fuerzas evolutivas. La población evaluada presentó un promedio de heterocigosidad esperada de 0,572 y la heterocigosidad observada fue menor (0,058). El índice de fijación (F) presentó un promedio total de 0,906, indicando que en Montería hay un exceso de homocigotos. Los valores de PIC permitieron determinar que los marcadores mPgCIR9, mPgCIR11, mPgCIR13, mPgCIR16, mPgCIR19 y mPgCIR22 son altamente informativos, mientras que mPgCIR23 es medianamente informativo. La población de guayaba en Montería presentó una baja diversidad genética dentro de las subpoblaciones, lo cual podría estar relacionado con eventos de endogamia como la autopolinización.