Publicación: Detección de paramyxovirus en murciélagos del departamento de Córdoba, Colombia
dc.contributor.advisor | Echeverri de la Hoz, Daniel Mauricio | |
dc.contributor.advisor | Martínez Bravo, Caty Milena | |
dc.contributor.author | Gómez Navarro, Nayerlys | |
dc.contributor.jury | Pérez Cogollo, Luis Carlos | |
dc.contributor.jury | Calderón Rangel, Alfonso | |
dc.date.accessioned | 2025-09-03T16:31:21Z | |
dc.date.available | 2025-09-03T16:31:21Z | |
dc.date.issued | 2025-09-02 | |
dc.description.abstract | Este estudio tuvo como objetivo detectar la presencia de paramyxovirus en murciélagos del departamento de Córdoba, Colombia. Se analizaron muestras de 100 individuos pertenecientes a 11 especies de la familia Phyllostomidae, recolectadas en zonas urbanas, periurbanas y rurales. Se emplearon técnicas de biología molecular, utilizando RT-PCR semianidada dirigida al gen L del virus. La detección se confirmó por electroforesis en gel de agarosa. Se identificó un caso positivo en una hembra adulta de Phyllostomus discolor capturada en una zona rural del municipio de Chimá. Esta especie, de hábitos omnívoros y amplia capacidad de movimiento, podría haber facilitado su exposición al virus en distintos entornos. A pesar de la baja frecuencia, este hallazgo representa el primer reporte de paramyxovirus en murciélagos en Colombia. Los resultados destacan la necesidad de fortalecer la vigilancia epidemiológica de virus emergentes en fauna silvestre, ampliar el esfuerzo de muestreo y aplicar herramientas moleculares más sensibles. Además, se sugiere explorar el rol ecológico de los murciélagos en la diseminación viral, especialmente en ecosistemas compartidos con otras especies como las aves migratorias. | spa |
dc.description.abstract | This study aimed to detect the presence of paramyxovirus in bats from the department of Córdoba, Colombia. Samples from 100 individuals belonging to 11 species of the Phyllostomidae family were analyzed, collected in urban, peri-urban, and rural areas. Molecular biology techniques were used, employing semi-nested RT-PCR targeting the L gene of the virus. Detection was confirmed by agarose gel electrophoresis. One positive case was identified in an adult female Phyllostomus discolor captured in a rural area of the municipality of Chimá. This species, with omnivorous habits and high mobility, could have facilitated its exposure to the virus in different environments. Despite the low frequency, this finding represents the first report of paramyxovirus in bats in Colombia. The results highlight the need to strengthen the epidemiological surveillance of emerging viruses in wildlife, expand sampling efforts, and apply more sensitive molecular tools. In addition, it is suggested to explore the ecological role of bats in viral dissemination, especially in ecosystems shared with other species such as migratory birds. | eng |
dc.description.degreelevel | Pregrado | |
dc.description.degreename | Médico(a) Veterinario(a) y Zootecnia | |
dc.description.modality | Trabajos de Investigación y/o Extensión | |
dc.description.tableofcontents | 1. Introducción .................13 | |
dc.description.tableofcontents | 2. Objetivos........................16 | |
dc.description.tableofcontents | 2.1 Objetivo general ...................................16 | |
dc.description.tableofcontents | 2.2 Objetivos específicos ..................................16 | |
dc.description.tableofcontents | 3. Marco Teórico.............................17 | |
dc.description.tableofcontents | 3.1 Murciélagos .....................................17 | |
dc.description.tableofcontents | 3.2 Murciélagos en el mantenimiento de virus .................18 | |
dc.description.tableofcontents | 3.3 Paramixovirus ...................20 | |
dc.description.tableofcontents | 3.3.1 Clasificación y Taxonomía....................20 | |
dc.description.tableofcontents | 3.3.2 Genoma y virión ……………..20 | |
dc.description.tableofcontents | 3.3.3 Sistema inmune del murciélago contra los paramixovirus ..........22 | |
dc.description.tableofcontents | 3.4 Antecedentes .......................22 | |
dc.description.tableofcontents | 3.4.1 Paramixovirus en murciélagos del viejo mundo..................22 | |
dc.description.tableofcontents | 3.4.2 Paramixovirus en murciélagos del nuevo mundo ............23 | |
dc.description.tableofcontents | 4. Metodología................................. 26 | |
dc.description.tableofcontents | 4.1 Localización .........................26 | |
dc.description.tableofcontents | 4.2 Tipo de estudio ...............................26 | |
dc.description.tableofcontents | 4.3 Componentes éticos.....................26 | |
dc.description.tableofcontents | 4.4 Procedimiento para la obtención de muestras..........26 | |
dc.description.tableofcontents | 4.5 Metodología para el cumplimiento del objetivo específico 2.2.1 ..... 27 | |
dc.description.tableofcontents | 4.5.1 Caracterización de la población de estudio ...............27 | |
dc.description.tableofcontents | 4.6 Metodología para el cumplimiento del objetivo específico 2.2.2 .............28 | |
dc.description.tableofcontents | 4.6.1 Extraccion de material genético................28 | |
dc.description.tableofcontents | 4.6.2 Detección molecular...................29 | |
dc.description.tableofcontents | 4.7 Metodología para el cumplimiento del objetivo específico 2.2.3 .....30 | |
dc.description.tableofcontents | 4.7.1 Distribución geográfica de los murciélagos positivos a paramixovirus….30 | |
dc.description.tableofcontents | 5. Resultados.......................31 | |
dc.description.tableofcontents | 5.1 Resultados relacionados con el cumplimiento del objetivo específico número 2.2.1..........................31 | |
dc.description.tableofcontents | 5.1.1 Caracterización de la población de estudio..............31 | |
dc.description.tableofcontents | 5.2 Resultados relacionados con el cumplimiento del objetivo específico número 2.2.2.......................................35 | |
dc.description.tableofcontents | 5.2.1 Extracción de material genético......................35 | |
dc.description.tableofcontents | 5.2.2 Detección molecular.........................35 | |
dc.description.tableofcontents | 5.3 Resultados relacionados con el cumplimiento del objetivo específico número 2.2.3.................................36 | |
dc.description.tableofcontents | 5.3.1 Distribución geográfica del murciélago positivo a paramixovirus......36 | |
dc.description.tableofcontents | 6. Discusión .................................39 | |
dc.description.tableofcontents | 7. Conclusiones ...................43 | |
dc.description.tableofcontents | 8. Recomendaciones..............................43 | |
dc.description.tableofcontents | 9. Referencias bibliográficas...............................44 | |
dc.format.mimetype | application/pdf | |
dc.identifier.instname | Universidad de Córdoba | |
dc.identifier.reponame | Repositorio Universidad de Córdoba | |
dc.identifier.repourl | https://repositorio.unicordoba.edu.co/ | |
dc.identifier.uri | https://repositorio.unicordoba.edu.co/handle/ucordoba/9544 | |
dc.language.iso | spa | |
dc.publisher | Universidad de Córdoba | |
dc.publisher.faculty | Facultad de Medicina Veterinaria y Zootecnia | |
dc.publisher.place | Berástegui, Córdoba, Colombia | |
dc.publisher.program | Medicina Veterinaria y Zootecnia | |
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dc.rights | Copyright Universidad de Córdoba, 2025 | |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | |
dc.rights.coar | http://purl.org/coar/access_right/c_abf2 | |
dc.rights.license | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.keywords | RT-PCR | eng |
dc.subject.keywords | Zoonosis | eng |
dc.subject.keywords | Wildlife | eng |
dc.subject.keywords | Epidemiological Surveillance | eng |
dc.subject.keywords | Emerging viruses | eng |
dc.subject.proposal | RT-PCR | spa |
dc.subject.proposal | Zoonosis | spa |
dc.subject.proposal | Fauna silvestre | spa |
dc.subject.proposal | Vigilancia epidemiológica | spa |
dc.subject.proposal | Virus emergentes | spa |
dc.title | Detección de paramyxovirus en murciélagos del departamento de Córdoba, Colombia | spa |
dc.type | Trabajo de grado - Pregrado | |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | |
dc.type.content | Text | |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | |
dspace.entity.type | Publication |
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