Publicación: Criopreservación de semen de bagre sapo Pseudopimelodus atricaudus
dc.contributor.advisor | Atencio García, Victor julio | |
dc.contributor.advisor | Espinosa Araujo, José Alonso | |
dc.contributor.author | Guzmán Yance, Miguel Ángel | |
dc.contributor.jury | Prieto Mojica, Camilo Alberto | |
dc.contributor.jury | Medina Robles, Víctor Mauricio | |
dc.date.accessioned | 2025-06-27T19:28:38Z | |
dc.date.available | 2028-06-27 | |
dc.date.available | 2025-06-27T19:28:38Z | |
dc.date.issued | 2025-06-27 | |
dc.description.abstract | El bagre sapo Pseudopimelodus atricaudus es una especie recientemente re-descrita taxonómicamente, distribuida en una de las cuencas hidrográficas más importantes de Colombia (Magdalena-Cauca). Sin embargo, esta cuenca enfrenta alta presión pesquera y deterioro ambiental, entre otros problemas de orden antrópico. Debido que sus hábitats están degradados se requiere estrategias para la conservación de esta especie. La crioconservación es una herramienta biotecnológica que permite la preservación del recurso genético. Esta investigación se llevó a cabo en el Instituto de Investigación Piscícola de la Universidad de Córdoba-CINPIC. Se utilizaron ejemplares de bagre sapo (n=18) provenientes del Bajo del río Cauca. El objetivo fue evaluar la calidad del semen criopreservado de P. atricaudus con etilenglicol (EG) y metanol (ME) a diferentes niveles de inclusión. En ensayos independientes se evaluaron dos protocolos de crioconservación. En el protocolo 1, se evaluó EG a cuatro niveles de inclusión (6, 8, 10 y 12%) combinado con leche en polvo descremada (LPD) al 3%. En el protocolo 2, se evaluó ME al 10% combinado con LPD incluida a tres niveles de inclusión de LPD (5, 10 o 15%). En ambos protocolos se utilizó glucosa al 6%. El semen se empacó en pajillas de 0.25 mL y se congeló en vapores de nitrógeno en un dry shipper durante 30 minutos. Se evaluó movilidad total, tipos de movilidad, velocidades espermáticas, tiempo de activación y concentración del semen fresco, precongelado y descongelado con sistema CASA (Microptic, SCA, España). La morfología de los espermatozoides fue descrita con ayuda de microscopia de barrido electrónico (SEM). La movilidad total del semen descongelado se redujo entre 54-70% en los dos protocolos evaluados, oscilando entre 41.9±3.4 y 47.0±5.5%. Los resultados sugieren que EG incluido al 6 o 10% con LPD al 3% y ME incluido al 10% con LPD al 5 o 10% diluidos en glucosa 6% son posibles soluciones crioprotectoras para la criopreservación de semen Pseudopimelodus atricaudus. Los espermatozoides de bagre sapo son uniflagelado, cabeza esférica con diámetro (1.51-1.99 µm) y longitud total (32.58-34.27 µm) similar a otros Siluriformes. Sin embargo, es necesario continuar evaluando otros protocolos para optimizar la crioconservación de P. atricaudus; así como la evaluación de su capacidad fecundante. | spa |
dc.description.abstract | The catfish Pseudopimelodus atricaudus is a recently taxonomically re-described species distributed in one of Colombia's most important watersheds (Magdalena-Cauca). However, this watershed faces high fishing pressure and environmental degradation, among other anthropogenic problems. Due to its degraded habitats, conservation strategies for this species are required. Cryopreservation is a biotechnological tool that allows for the preservation of genetic resources. This research was conducted at the Fish Research Institute of the University of Córdoba (CINPIC). Catfish (n=18) from the Lower Cauca River were used. The objective was to evaluate the quality of cryopreserved P. atricaudus semen with ethylene glycol (EG) and methanol (ME) at different inclusion levels. Two cryopreservation protocols were evaluated in independent trials. In protocol 1, EG was assessed at four inclusion levels (6, 8, 10, and 12%) combined with 3% skimmed milk powder (SMP). Protocol 2 included 10% EM combined with SMP at three inclusion levels (5, 10, or 15%). 6% glucose was used in both protocols. Semen was packaged in 0.25 mL straws and frozen under nitrogen vapor in a dry shipper for 30 minutes. Total motility, motility types, sperm velocities, activation time, and concentration of fresh, pre-frozen, and thawed semen were assessed using a CASA system (Microptic, SCA, Spain). Sperm morphology was described using scanning electron microscopy (SEM). Total motility of thawed semen was reduced by 54–70% in both protocols evaluated, ranging from 41.9 ± 3.4 to 47.0 ± 5.5%. The results suggest that 6% or 10% EG embedded with 3% LPD and 10% ME embedded with 5% or 10% LPD diluted in 6% glucose are potential cryoprotective solutions for cryopreservation of Pseudopimelodus atricaudus semen. Catfish sperm are uniflagellate, with a spherical head, with a diameter (1.51–1.99 µm) and total length (32.58–34.27 µm) comparable to other siluriformids. However, further evaluation of other protocols is necessary to optimize P. atricaudus cryopreservation and assess its fertilization capacity. | eng |
dc.description.degreelevel | Maestría | |
dc.description.degreename | Magíster en Acuicultura Tropical | |
dc.description.modality | Trabajos de Investigación y/o Extensión | |
dc.description.tableofcontents | RESUMEN | spa |
dc.description.tableofcontents | ABSTRACT | spa |
dc.description.tableofcontents | INTRODUCCIÓN | spa |
dc.description.tableofcontents | OBJETIVOS | spa |
dc.description.tableofcontents | Objetivo general | spa |
dc.description.tableofcontents | Objetivos específicos | spa |
dc.description.tableofcontents | MARCO TEORICO | spa |
dc.description.tableofcontents | Generalidades de Pseudopimelodus atricaudus | spa |
dc.description.tableofcontents | Características de los espermatozoides en teleósteos | spa |
dc.description.tableofcontents | Crioconservación de semen en peces | spa |
dc.description.tableofcontents | Crioprotectores | spa |
dc.description.tableofcontents | Osmolaridad | spa |
dc.description.tableofcontents | Criopreservación de semen de peces en Colombia | spa |
dc.description.tableofcontents | MATERIALES Y METODOS | spa |
dc.description.tableofcontents | Localización y área de estudio | spa |
dc.description.tableofcontents | Material biológico | spa |
dc.description.tableofcontents | Cinética espermática | spa |
dc.description.tableofcontents | Crioconservación de semen | spa |
dc.description.tableofcontents | Osmolaridad | spa |
dc.description.tableofcontents | Análisis estadísticos | spa |
dc.description.tableofcontents | Consideraciones éticas | spa |
dc.description.tableofcontents | RESULTADOS | spa |
dc.description.tableofcontents | Morfología de espermatozoides | spa |
dc.description.tableofcontents | Características generales del semen de P. atricaudus | spa |
dc.description.tableofcontents | Osmolaridad | spa |
dc.description.tableofcontents | Cinética espermática | spa |
dc.description.tableofcontents | DISCUSIÓN | spa |
dc.description.tableofcontents | Morfología del espermatozoide | spa |
dc.description.tableofcontents | Osmolaridad | spa |
dc.description.tableofcontents | Cinética semen fresco, precongelado y descongelado | spa |
dc.description.tableofcontents | CONCLUSIÓN | spa |
dc.description.tableofcontents | REFERENCIAS | spa |
dc.format.mimetype | application/pdf | |
dc.identifier.instname | Universidad de Córdoba | |
dc.identifier.reponame | Repositorio Universidad de Córdoba | |
dc.identifier.repourl | https://repositorio.unicordoba.edu.co/ | |
dc.identifier.uri | https://repositorio.unicordoba.edu.co/handle/ucordoba/9215 | |
dc.language.iso | spa | |
dc.publisher | Universidad de Córdoba | |
dc.publisher.faculty | Facultad de Medicina Veterinaria y Zootecnia | |
dc.publisher.place | Montería, Córdoba, Colombia | |
dc.publisher.program | Maestría en Acuicultura Tropical | |
dc.relation.references | Alavi, S. M. H., Rodina, M., Policar, T., & Linhart, O. (2009). Relationship between semen characteristics and body size in Barbus barbus L. (Teleostei: Cyprinidae) and effects of ions and osmolality on sperm motility. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 153(4), 430–437. https://doi.org/10.1016/j.cbpa.2009.04.001 | |
dc.relation.references | Albarracín, J. (2005). Vitrificación de ovocitos bovinos mediante la técnica open pulled straw: Estudio estructural de cromosomas, microtúbulos y microfilamentos y posterior desarrollo embrionario in vitro [Tesis doctoral, Universidad Autónoma de Barcelona]. | |
dc.relation.references | Andrade, J., & Olaya, A. (2023). Impactos ambientales asociados a las hidroeléctricas en Colombia. Revista De Investigación Agraria Y Ambiental, 14(2), 217-250. https://doi.org/10.22490/21456453.6074 | |
dc.relation.references | Ashwood-Smith, M. J., & Lough, P. (1975). Cryoprotection of mammalian cells in tissue culture with methanol. Cryobiology, 12(5), 517–518. https://doi.org/10.1016/0011-2240(75)90034-6 | |
dc.relation.references | Atencio-García, V. J., Cabrales-Hessen, S. S., & Espinosa-Araujo, J. A. (2021). Efecto de etilenglicol y leche en polvo en la criopreservación de semen de bocachico (Prochilodus magdalenae). Revista Colombiana de Biotecnología, 23(2), 25–35. https://doi.org/10.15446/rev.colomb.biote.v23n2.91188 | |
dc.relation.references | Atencio-García, V. J., Montes-Petro, C. D., Tapia-Pacheco, C. J., Madariaga-Mendoza, D. L., & Espinosa-Araujo, J. A. (2024, 8–10 de mayo). Efecto de diferentes inductores hormonales en la calidad seminal de bagre sapo Pseudopimelodus atricaudus [Póster]. XVII Congreso Colombiano de Ictiología & VIII Encuentro Suramericano de Ictiólogos, Universidad de los Llanos, Villavicencio, Colombia. | |
dc.relation.references | Atencio-García, V., Dorado-Longas, M., Montes-Petro, C., Prieto-Guevara, M., & Espinosa-Araujo, J. (2017). Cryopreservation of dorada (Brycon moorei) sperm with dimethyl sulfoxide. Revista Colombiana de Biotecnología, 19(2), 87–94. https://doi.org/10.15446/rev.colomb.biote.v19n2.59987 | |
dc.relation.references | Atencio-García, V., García Arteaga, Y., Pérez Morales, J., Pardo-Carrasco, S., & Prieto-Guevara, M. (2014). Efecto de la densidad de siembra en el manejo de la primera alimentación de larvas de bagre blanco Sorubim cuspicaudus. Revista Científica Sabia, 1(3), 25–38. http://www.unipacifico.edu.co:8095/publicacionesunipa/documentos/art_sb3_3.pdf | |
dc.relation.references | Atencio-García, V., Padilla-Izquierdo, D., Robles-González, J., Prieto-Guevara, M., Pardo-Carrasco, S., & Espinosa-Araujo, J. (2023). Damage to Sorubim cuspicaudus sperm cryopreserved with ethylene glycol. Animals, 13(2), 235. https://doi.org/10.3390/ani13020235 | |
dc.relation.references | Ávila-Portillo, L. M., Madero, J. I., López, C., León, M. F., Acosta, L., Gómez, C., Delgado, L. G., Gómez, C., Lozano, J. M., & Reguero, M. T. (2006). Fundamentos de criopreservación. Revista Colombiana de Obstetricia y Ginecología, 57(4), 291–300. https://doi.org/10.18597/rcog.468 | |
dc.relation.references | Barros-Barrios, O. J., Suárez-Martínez, R. O., Gómez-Ramírez, E., Guaje-Ramírez, D. N., & Medina-Robles, V. M. (2025). Sperm Quality, Milt Biochemistry, and Cellular Ultrastructure in Fresh and Cryopreserved Sperm of Brycon amazonicus. Aquaculture Research, 2025(1). https://doi.org/10.1155/are/6671651 | |
dc.relation.references | Bearden, H., & Fuquay, J. (1982). Reproducción animal aplicada. El Manual Moderno. | |
dc.relation.references | Benson, J. D. (2015). Modeling and optimization of cryopreservation. En W. F. Wolkers & H. Oldenhof (Eds.), Cryopreservation and freeze-drying protocols (pp. 83–120). Humana Press. https://doi.org/10.1007/978-1-4939-2193-5 | |
dc.relation.references | Bernáth, G., Csorbai, B., Nagy, B., Csókás, E., Molnár, J., Bartucz, T., Láng, Z., Gyurcsák, M., Hegyi, Á., Kobolák, J., Griffitts, J., Ferincz, Á., Urbányi, B., & Bokor, Z. (2023). The investigation of post-thaw chilled storage and the applicability of large-scale cryopreservation in chub (Squalius cephalus) sperm. Cryobiology, 113, 1–9. https://doi.org/10.1016/j.cryobiol.2023.104588 | |
dc.relation.references | Billard, R., Linhart, O., Fierville, F., & Cosson, J. (1997). Motility of European catfish (Silurus glanis) spermatozoa in testes and milt. Polskie Archiwum Hydrobiologii, 44, 115–122. | |
dc.relation.references | Bobe, J., & Labbé, C. (2010). Egg and sperm quality in fish. General and Comparative Endocrinology, 165(3), 535–548. https://doi.org/10.1016/j.ygcen.2009.02.011 | |
dc.relation.references | Cabrita, E., Martínez-Páramo, S., Gavaia, P., Riesco, M., Valcarce, D., Sarasquete, C., Herráez, M., & Robles, V. (2014). Factors enhancing fish sperm quality and emerging tools for sperm analysis. Aquaculture, 432, 389–401. https://doi.org/10.1016/j.aquaculture.2014.04.034 | |
dc.relation.references | Cabrita, E., Sarasquete, C., Martínez-Páramo, S., Robles, V., Beirão, J., Pérez-Cerezales, S., & Herráez, M. (2010). Cryopreservation of fish sperm: applications and perspectives. Journal of Applied Ichthyology, 26, 623–635. https://doi.org/10.1111/j.1439-0426.2010.01556.x | |
dc.relation.references | Cano Cuello, R. R. (2020). Efecto del volumen de empaque, el tiempo y temperatura de descongelación en la calidad del semen criopreservado de bocachico Prochilodus magdalenae [Tesis de maestría, Universidad de Córdoba]. https://repositorio.unicordoba.edu.co/server/api/core/bitstreams/6196540f-2d94-4322-91fb-4e5b6d08dffa/content | |
dc.relation.references | Carneiro, P., Azevedo, H. C., Santos, J. P., & Alexandre, M. (2012). Cryopreservation of tambaqui (Colossoma macropomum) semen: Extenders, cryoprotectants, dilution ratios and freezing methods. CryoLetters, 33(5), 385–393. | |
dc.relation.references | Choez, K. (2016). Determinación de la concentración óptima de distintos agentes crioprotectores para la criopreservación de espermatozoides epididimarios de alpaca [Tesis de maestría, Universidad Nacional Mayor de San Marcos]. | |
dc.relation.references | Ciereszko, A., Dietrich, M., & Nynca, J. (2017). Fish semen proteomics: New opportunities in fish reproductive research. Aquaculture, 472, 81–92. https://doi.org/10.1016/j.aquaculture.2016.03.005 | |
dc.relation.references | Cosson, J., Billard, R., Cibert, C., & Dréanno, C. (1999). Factores iónicos que regulan la motilidad del esperma de pescado. En C. Gagnon (Ed.), El gameto masculino: Del conocimiento básico a las aplicaciones clínicas (pp. 161–186). Cache River Press. | |
dc.relation.references | Cosson, M., Cosson, J., & Billard, R. (1991). cAMP dependence of movement initiation in intact and demembranated trout spermatozoa. Bulletin of the Institute of Zoology, Academia Sinica, 16, 263–266. | |
dc.relation.references | Cosson, M., Cosson, J., Andrè, F., & Billard, R. (1995). cAMP/ATP relationship in the activation of trout sperm motility: Their interaction in membrane‐deprived models and in live spermatozoa. Cell Motility, 31(2), 159–176. https://doi.org/10.1002/cm.970310208 | |
dc.relation.references | Costa, R. S., Souza, F. M. S., Senhorini, J. A., Ribeiro, D. C., Bashiyo-Silva, C., Coelho, G. C. Z., Verissimo-Silveira, R., & Ninhaus-Silveira, A. (2018). Improved solution for vitrification of Prochilodus lineatus embryos based on the reduction in risk factors: Toxicity, osmotic responses and ice-nucleation. Aquaculture Research, 49(2), 793–800. https://doi.org/10.1111/are.13510 | |
dc.relation.references | Cruz-Casallas, P., Pardo-Carrasco, S., Arias-Castellanos, J., Lombo-Castellanos, P., Lombo-Rodríguez, D., & Pardo-Mariño, J. (2004). Cryopreservation of yamú (Brycon siebenthalae) milt. Journal of the World Aquaculture Society, 35(4), 529–535. | |
dc.relation.references | Da Costa, B., Lassen, P., & Streit, P. (2024). Cryopreservation-induced morphological changes in freshwater fish sperm: A systematic review. Biopreservation and Biobanking. https://doi.org/10.1089/bio.2023.0008 | |
dc.relation.references | Dietrich, M. A., Arnold, G. J., Fröhlich, T., Otte, K. A., Dietrich, G. J., & Ciereszko, A. (2015). Proteomic analysis of extracellular medium of cryopreserved carp (Cyprinus carpio L.) semen. Comparative Biochemistry and Physiology Part D: Genomics & Proteomics, 15, 49–57. https://doi.org/10.1016/j.cbd.2015.05.003 | |
dc.relation.references | DoNascimiento, C., Bogotá Gregory, J. D., Albornoz Garzón, J. G., Méndez López, A., Villa Navarro, F. A., Herrera Collazos, E. E., Agudelo Zamora, H., Arce, H. M., Herrera-Collazos, E. E., & Maldonado-Ocampo, J. A. (2021). Lista de especies de peces de agua dulce de Colombia / Checklist of the freshwater fishes of Colombia. Asociación Colombiana de Ictiólogos | |
dc.relation.references | Duarte, L. O., García, E., Tejeda, K., Cuello, F., Gil-Manrique, B., De León, G., Curiel, J., Cuervo, C., Vargas, O., Isaza, E., Manjarrés–Martínez, L., & Reyes-Ardila, H. (2022). Estadísticas de desembarco y esfuerzo de las pesquerías artesanales de Colombia – Enero a octubre de 2022. Autoridad Nacional de Acuicultura y Pesca (AUNAP) & Universidad del Magdalena | |
dc.relation.references | Duque-Escobar, G. (2019). El río Cauca en el desarrollo de la región. Universidad Nacional de Colombia. https://repositorio.unal.edu.co/bitstream/handle/unal/77027/elriocaucaeneldesarrollodelaregion.pdf | |
dc.relation.references | Dzyuba, B., Bondarenko, O., Fedorov, P., Gazo, I., Prokopchuk, G., & Cosson, J. (2017). Energetics of fish spermatozoa: The proven and the possible. Aquaculture, 472, 60–72. https://doi.org/10.1016/j.aquaculture.2016.05.038 | |
dc.relation.references | Dzyuba, V., & Cosson, J. (2014). Motility of fish spermatozoa: From external signaling to flagella response. Reproductive Biology, 14(3), 165–175. https://doi.org/10.1016/j.repbio.2013.12.005 | |
dc.relation.references | Eddy, E. M., & O’Brien, D. A. (1994). The spermatozoon. En E. Knobil & J. D. Neill (Eds.), The physiology of reproduction (pp. 29–77). Raven Press. | |
dc.relation.references | El Kamouh, M., Brionne, A., Sayyari, A., Laurent, A., & Labbé, C. (2023). Cryopreservation effect on DNA methylation profile in rainbow trout spermatozoa. Scientific Reports, 13(1), 1–20. https://doi.org/10.1038/s41598-023-44803-2 | |
dc.relation.references | Espinosa, A. (2013). Estandarización de un protocolo para la crioconservación de semen de bagre blanco Sorubim cuspicaudus [Tesis de maestría, Universidad de Córdoba]. | |
dc.relation.references | Fornari, E., Grandi, S., & Fornari, D. (2011). Effects of intra-brand competition between private labels and manufacturer brands: Empirical results from the Italian market. The International Review of Retail, Distribution and Consumer Research, 21(5), 541–554. https://doi.org/10.1080/09593969.2011.620132 | |
dc.relation.references | Frits, M., & Baren, M. (2000). Dynamics of the mammalian sperm plasma membrane in the process of fertilization. Biochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1469(3), 197–235. https://doi.org/10.1016/S0304-4157(00)00018-6 | |
dc.relation.references | Fu, S., Jiang, J., Yang, W., & Zhu, J. (2016). A histological study of testis development and ultrastructural features of spermatogenesis in cultured Acrossocheilus fasciatus. Tissue & Cell, 48(1), 49–62. https://doi.org/10.1016/j.tice.2015.10.005 | |
dc.relation.references | Glogowski, J., Babiak, I., Kucharczyk, D., Luczynski, M., & Piros, B. (1999). Some properties of bream (Abramis brama L.) sperm and its cryopreservation. Aquaculture Research, 30(10), 765–772. https://doi.org/10.1046/j.1365-2109.1999.00399.x | |
dc.relation.references | Gonçalves, L., Silva, G., Viana, I., Hainfellner, P., Ferreira, M., Batlouni, S., & Rocha, R. (2019). Testicular structure and development of germ cells of Hypophthalmus marginatus Valenciennes 1840 (Siluriformes: Pimelodidae). Animal Reproduction Science, 211, 1–12. https://doi.org/10.1016/j.anireprosci.2019.106223 | |
dc.relation.references | Guthrie, H. D., Liu, J., & Critser, J. K. (2002). Osmotic tolerance limits and effects of cryoprotectants on motility of bovine spermatozoa. Biology of Reproduction, 67(6), 1811–1816. https://doi.org/10.1095/biolreprod67.6.1811 | |
dc.relation.references | Harvey, B. (1993). Cryopreservation of fish spermatozoa. En Genetic Conservation of Salmonid Fishes (pp. 175–179). Springer US. https://doi.org/10.1007/978-1-4615-2866-1_12 | |
dc.relation.references | Hayashi, H., Yamamoto, K., Yonekawa, H., & Morisawa, M. (1987). Involvement of tyrosine protein kinase in the initiation of flagellar movement in rainbow trout spermatozoa. The Journal of Biological Chemistry, 262(34), 16692–16698 | |
dc.relation.references | Herráez, M. P., Ausió, J., Devaux, A., González-Rojo, S., Fernández-Díez, C., Bony, S., Saperas, N., & Robles, V. (2017). Paternal contribution to development: Sperm genetic damage and repair in fish. Aquaculture, 472, 45–59. https://doi.org/10.1016/j.aquaculture.2016.03.007 | |
dc.relation.references | Herrera-Cruz, E., Aristizabal-Regino, J., Yepes-Blandón, J., Estrada-Posada, A., Espinosa-Araujo, J., & Atencio-García, V. (2019). Criopreservación de semen de bagre rayado Pseudoplatystoma magdaleniatum con tres diferentes crioprotectores. Revista Colombiana de Biotecnología, 21(2), 55–62. | |
dc.relation.references | Inaba, K. (2007). Molecular mechanisms of the activation of flagellar motility in sperm. En S. M. H. Alavi, J. Cosson, K. Coward, & G. Rafiee (Eds.), Fish spermatology (pp. 267–279). Alpha Science Inc. | |
dc.relation.references | Islam, M., & Akhter, T. (2011). Tale of fish sperm and factors affecting sperm motility: A review. Advances in Life Sciences, 1, 11–19. https://doi.org/10.5923/j.als.20110101.03 | |
dc.relation.references | Jamieson, B. (1991). Fish evolution and systematics: Evidence from spermatozoa. Cambridge University Press. https://doi.org/10.1017/S0025315400053625 | |
dc.relation.references | Jiménez-Segura, L., & Lasso, C. A. (Eds.). (2021). Peces de la cuenca del río Magdalena, Colombia: diversidad, conservación y uso sostenible. Instituto de Investigación de Recursos Biológicos Alexander von Humboldt. https://doi.org/10.21068/A2020RRHHXIX | |
dc.relation.references | Jiménez-Segura, L., Restrepo-Santamaría, D., López-Casas, S., Valderrama, M., Álvarez, J., & Gómez, D. (2014). Ictiofauna y desarrollo del sector hidroeléctrico en la cuenca del río Magdalena-Cauca, Colombia. Biota Colombiana, 15(2), 3–25. https://www.redalyc.org/pdf/491/49140782002.pdf | |
dc.relation.references | Judycka, S., Dietrich, M., Szczepkowska, B., Szczepkowski, B., Liszewska, E., & Ciereszko, A. (2024). Evaluation of the effects of glucose concentration in extender and sperm concentration in straw for cryopreservation of Atlantic sturgeon (Acipenser oxyrinchus) semen. Aquaculture Reports, 35, 1–7. https://doi.org/10.1016/j.aqrep.2024.101995 | |
dc.relation.references | Kumar, J. S. S., & Betsy, C. J. (2015). Cryopreservation of fish gametes and its role in enhancing aquaculture production. En Advances in Marine and Brackishwater Aquaculture (pp. 241–246). Springer India. https://doi.org/10.1007/978-81-322-2271-2_22 | |
dc.relation.references | Li, P., Wei, Q., & Liu, L. (2008). DNA integrity of Polyodon spathula cryopreserved sperm. Journal of Applied Ichthyology, 24(2), 121–125. https://doi.org/10.1111/j.1439-0426.2007.01025.x | |
dc.relation.references | Li, Z. L., Lin, Q. L., Liu, R. J., Xie, W. Y., & Xiao, W. F. (2007). Zhonghua Yi Xue Za Zhi, 87(45), 3174–3177. | |
dc.relation.references | Linhart, O., Rodina, M., Flajshans, M., Gela, D., & Kocour, M. (2005). Cryopreservation of European catfish Silurus glanis sperm: Sperm motility, viability, and hatching success of embryos. Cryobiology, 51(3), 250–261. https://doi.org/10.1016/j.cryobiol.2005.07.005 | |
dc.relation.references | López-Hernández, J. C. (2017). Biología, fisiología espermática básica y efecto de la radiación ultravioleta sobre la fisiología e integridad de las células espermáticas del bagre tropical Rhamdia laticauda [Tesis de maestría no publicada]. Universidad Juárez Autónoma de Tabasco. | |
dc.relation.references | López-Hernández, J. C. (2017). Biología, fisiología espermática básica y efecto de la radiación ultravioleta sobre la fisiología e integridad de las células espermáticas del bagre tropical Rhamdia laticauda [Tesis de maestría no publicada]. Universidad Juárez Autónoma de Tabasco. | |
dc.relation.references | Márián, T., Krasznai, Z., Balkay, L., Balázs, M., Emri, M., Bene, L., & Trón, L. (1993). Hypo-osmotic shock induces an osmolality-dependent permeabilization and structural changes in the membrane of carp sperm. Journal of Histochemistry & Cytochemistry, 41(2), 291–297. https://doi.org/10.1177/41.2.8419464 | |
dc.relation.references | Marín, S., Chiang, K., Bassilian, S., Lee, W.-N. P., Boros, L. G., Fernández-Novell, J. M., Centelles, J. J., Medrano, A., Rodriguez-Gil, J. E., & Cascante, M. (2003). Metabolic strategy of boar spermatozoa revealed by a metabolomic characterization. FEBS Letters, 554(3), 342–346. https://doi.org/10.1016/S0014-5793(03)01185-2 | |
dc.relation.references | Marrugo-Negrete, J., Vargas-Licona, S., Ruiz-Guzmán, J. A., Marrugo-Madrid, S., Bravo, A. G., & Díez, S. (2020). Human health risk of methylmercury from fish consumption at the largest floodplain in Colombia. Environmental Research, 182, 109050. https://doi.org/10.1016/j.envres.2019.109050 | |
dc.relation.references | Martínez, J. G., & Pardo-Carrasco, S. (2010). Crioconservación de semen en peces: Efectos sobre la movilidad espermática y la fertilidad. Acta Biológica Colombiana, 15(2), 3–23. | |
dc.relation.references | Martínez, J., Atencio-García, V., & Pardo-Carrasco, S. (2012). DNA fragmentation and membrane damage of bocachico Prochilodus magdalenae (Ostariophysi, Prochilodontidae) sperm following cryopreservation with dimethylsulfoxide and glucose. Neotropical Ichthyology, 10(3), 577–586. https://doi.org/10.1590/S1679-62252012005000018 | |
dc.relation.references | Martínez-Páramo, S., Horváth, Á., Labbé, C., Zhang, T., Robles, V., Herráez, P., Suquet, M., Adams, S., Viveiros, A., Tiersch, T. R., & Cabrita, E. (2017). Cryobanking of aquatic species. Aquaculture, 472, 156–177. https://doi.org/10.1016/j.aquaculture.2016.05.042 | |
dc.relation.references | Massip, A. (2001). Cryopreservation of embryos of farm animals. Reproduction in Domestic Animals, 36(2), 49–55. https://doi.org/10.1046/j.1439-0531.2001.00248.x | |
dc.relation.references | Medina-Robles, V. M., Duarte-Trujillo, A. S., & Cruz-Casallas, P. E. (2020). Crioconservación seminal en peces de agua dulce: Aspectos biotecnológicos, celulares y bioquímicos. Orinoquia, 24(2), 51–78. https://doi.org/10.22579/20112629.630 | |
dc.relation.references | Medina-Robles, V. M., Sandoval-Vargas, L. Y., Suárez-Martínez, R. O., Gómez-Ramírez, E., Guaje-Ramírez, D. N., & Cruz-Casallas, P. E. (2023). Cryostorage of white cachama (Piaractus orinoquensis) sperm: Effects on cellular, biochemical and ultrastructural parameters. Aquaculture Reports, 29, 101477. https://doi.org/10.1016/j.aqrep.2023.101477 | |
dc.relation.references | Medina-Robles, V. M., Velasco-Santamaría, Y. M., & Cruz-Casallas, P. E. (2005). Aspectos generales de la crioconservación espermática en peces teleósteos. Revista Colombiana de Ciencias Pecuarias, 18(1), 34–48. http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-06902005000100005&lng=en&tlng=es | |
dc.relation.references | Medina-Robles, V., Diana, N., Guaje-Ramírez, D., Marín-Cossio, L., Sandoval-Vargas, L., & Cruz-Casallas, P. (2019). Crioconservación seminal de Colossoma macropomum como estrategia de producción y conservación en la Orinoquia Colombiana. Orinoquia, 23(1), 15–24. https://doi.org/10.22579/20112629.537 | |
dc.relation.references | Medina-Robles, V., Velasco-Santamaría, Y. M., & Cruz-Casallas, P. E. (2006). Los bancos de recursos genéticos y su papel en la conservación de la biodiversidad. Orinoquia, 10(1), 71–77. https://doi.org/10.22579/20112629.205 | |
dc.relation.references | Melo-Maciel, M., Nunes, J., Pinheiro, J., Nunes, L., Pinheiro, R., Lopes, J., & Salmito-Vanderley, C. (2015). Sperm motility and velocities of Characiformes fishes in different times post-activation. Semina: Ciências Agrárias, 36(6), 4023–4030. https://doi.org/10.5433/1679-0359.2015v36n6p4023 | |
dc.relation.references | Meryman, H. T. (1968). Modified model for the mechanism of freezing injury in erythrocytes. Nature, 218(5139), 333–336. https://doi.org/10.1038/218333a0 | |
dc.relation.references | Müller, K., Labbé, C., & Zachowski, A. (1994). Phospholipid transverse asymmetry in trout spermatozoa plasma membrane. Biochimica et Biophysica Acta, 1192(1), 21–26. https://doi.org/10.1016/0005-2736(94)90138-4 | |
dc.relation.references | Neyrão, I. M., Santos, F. B. P., Rodrigues, R. B., Streit, D. P., Jr., & Godoy, L. (2024). Use of powdered milk in semen cryopreservation protocols for fish: A systematic review. Biopreservation and Biobanking, 22(1), 4–20. https://doi.org/10.1089/bio.2022.0091 | |
dc.relation.references | Nynca, J., Arnold, G., Fröhlich, T., & Ciereszko, A. (2015). Cryopreservation-induced alterations in protein composition of rainbow trout semen. Proteomics, 15, 2643–2654. https://doi.org/10.1002/pmic.201400525 | |
dc.relation.references | Ortega-Lara, A., Aguiño, A., & Sánchez, G. C. (2002). Los peces del Alto Cauca: Caracterización de la ictiofauna nativa de los principales ríos de la cuenca alta del río Cauca en el departamento del Cauca (Primera parte). Fundación para la Investigación y el Desarrollo Sostenible (FUNINDES) & Corporación Autónoma Regional del Cauca (CRC). | |
dc.relation.references | Osorio-Pérez, A. (2017). Fisiología y microestructura espermática del pejelagarto tropical Atractosteus tropicus [Tesis de licenciatura no publicada]. Universidad Juárez Autónoma de Tabasco. | |
dc.relation.references | Pardo-Carrasco, S., Salas-Villalva, J., Reza-Gaviria, L., Espinosa-Araújo, J., & Atencio-García, V. (2015). Criopreservación de semen de bagre blanco (Sorubim cuspicaudus) con dimetilacetamida como crioprotector. Revista CES Medicina Veterinaria y Zootecnia, 10(2), 122–131. https://revistas.ces.edu.co/index.php/mvz/article/view/3645/2453 | |
dc.relation.references | Pegg, D. E. (2010). The relevance of ice crystal formation for the cryopreservation of tissues and organs. Cryobiology, 60(3), S36–S44. https://doi.org/10.1016/j.cryobiol.2010.02.003 | |
dc.relation.references | Perry, C. T., Corcini, C. D., Anciuti, A. N., Otte, M. V., Soares, S. L., Garcia, J. R. E., Muelbert, J. R. E., & Varela, A. S. (2019). Amides as cryoprotectants for the freezing of Brycon orbignyanus sperm. Aquaculture, 508, 90–97. https://doi.org/10.1016/j.aquaculture.2019.03.015 | |
dc.relation.references | Pessoa, G., López, L., Rosero, J., Dos Santos, S., Yasui, G., Senhorini, J., & Monzani, P. (2024). Isolation and cryopreservation of Pseudopimelodus mangurus (Siluriformes) spermatogonial cells. Cryobiology, 104, 941. https://doi.org/10.1016/j.cryobiol.2024.104941 | |
dc.relation.references | Pinheiro, J. P. S., Leite-Castro, L. V., Oliveira, F. C. E., Linhares, F. R. A., Lopes, J. T., & Salmito-Vanderley, C. S. B. (2016). Qualidade do sêmen de tambaqui (Colossoma macropomum) criopreservado em diferentes concentrações de gema de ovo. Ciência Animal Brasileira, 17(2), 267–273. https://doi.org/10.1590/1089-6891v17i225386 | |
dc.relation.references | Polge, C., Smith, A. U., & Parkes, A. S. (1949). Revival of spermatozoa after vitrification and dehydration at low temperatures. Nature, 164(4172), 666. https://doi.org/10.1038/164666a0 | |
dc.relation.references | Psenicka, M., Alavi, S. M. H., Rodina, M., Gela, D., Nebesarova, J., & Linhart, O. (2007). Morphology and ultrastructure of Siberian sturgeon (Acipenser baerii) spermatozoa using scanning and transmission electron microscopy. Biology of the Cell, 99(2), 103–115. https://doi.org/10.1042/BC20060060 | |
dc.relation.references | Ramírez-Merlano, J., Medina-Robles, V., & Cruz-Casallas, P. (2011). Crioconservación seminal de bagre rayado Pseudoplatystoma metaense (Teleostei, Pimelodidae), bajo diferentes protocolos de congelación. Archivos de Medicina Veterinaria, 43, 135–144. https://doi.org/10.4067/S0301-732X2011000200006 | |
dc.relation.references | Ramírez-Merlano, J.A, Medina-Robles, V.M, & Cruz-Casallas, P.E. (2011). Crioconservación seminal de bagre rayado Pseudoplatystoma metaense (Teleostei, Pimelodidae), bajo diferentes protocolos de congelación. Archivos de medicina veterinaria, 43(2), 135-144. https://doi.org/10.4067/S0301-732X2011000200006 | |
dc.relation.references | Restrepo-Gómez, A. M., Rangel-Medrano, J. D., Márquez, E. J., & Ortega-Lara, A. (2020). Two new species of Pseudopimelodus Bleeker, 1858 (Siluriformes: Pseudopimelodidae) from the Magdalena Basin, Colombia. PeerJ, 8, e9723. https://doi.org/10.7717/peerj.9723 | |
dc.relation.references | Rodrigues, R. B., Uczay, M., Brito, V. B., Nunes-Fossati, A. A., Godoy, A. C., Moura, D. J., Vogel, C. I. G., Nogueira-Vasconcelos, A. C., & Streit, D. P. (2020). Skim milk powder used as a non-permeable cryoprotectant reduces oxidative and DNA damage in cryopreserved zebrafish sperm. Cryobiology, 93, 76–84. https://doi.org/10.1016/j.cryobiol.2020.10.005 | |
dc.relation.references | Rodríguez, C. (2009). Efecto del plasma seminal sobre la supervivencia de espermatozoides criopreservados de alpaca (Vicugna pacos) [Tesis de maestría, Universidad Nacional Mayor de San Marcos]. | |
dc.relation.references | Rurangwa, E., Kime, D. E., Ollevier, F., & Nash, J. P. (2004). The measurement of sperm motility and factors affecting sperm quality in cultured fish. Aquaculture, 234(1–4), 1–28. https://doi.org/10.1016/j.aquaculture.2003.12.006 | |
dc.relation.references | Sáez-Espinosa, P., Franco-Esclapez, C., Robles-Gómez, L., Silva, W. T. A. F., Romero, A., Immler, S., & Gómez-Torres, M. J. (2022). Morphological and ultrastructural alterations of zebrafish (Danio rerio) spermatozoa after motility activation. Theriogenology, 188, 108–115. https://doi.org/10.1016/j.theriogenology.2022.05.025 | |
dc.relation.references | Salmito-Vanderley, C. S. B., Almeida-Monteiro, P. S. D., & Nascimento, R. V. (2016). Tecnologia de conservação de sêmen de peixes: Resfriamento, congelação e uso de antioxidantes. Revista Brasileira de Reprodução Animal, 40(4), 194–199. | |
dc.relation.references | Sant’Anna, J., Moraes-Neto, A., Castro, J., Sczepanski, T., Pohv, J., Lopera-Barrero, N., & Artoni, R. (2019). Quantitative and qualitative variables of semen from surubim do Iguaçu, Steindachneridion melanodermatum Garavello, 2005 (Siluriformes: Pimelodidae). Brazilian Journal of Biology, 79(1), 1–5. https://doi.org/10.1590/1519-6984.09016 | |
dc.relation.references | Saudrais, C., Fierville, F., Loir, M., Le Rumeur, E., Cibert, C., & Cosson, J. (1998). The use of phosphocreatine plus ADP as energy source for motility of membrane-deprived trout spermatozoa. Cell Motility and the Cytoskeleton, 41(2), 91–106. https://doi.org/10.1002/(SICI)1097-0169(1998)41:2<91::AID-CM1>3.0.CO;2-I | |
dc.relation.references | Schulman, M. E., Cartelle, J. H., Malcervelli, D. M., Torres, P., Rivolta, M. Á., Cisale, H. O., & Fischman, M. L. (2024). Análisis morfométrico de espermatozoides de peces nativos de lagunas pampásicas. Biología Acuática, 42(en curso), 038. https://doi.org/10.24215/16684869e038 | |
dc.relation.references | Suquet, M., Dreanno, C., Fauvel, C., Cosson, J., & Billard, R. (2000). Cryopreservation of sperm in marine fish. Aquaculture Research, 31(3), 231–243. https://doi.org/10.1046/j.1365-2109.2000.00445.x | |
dc.relation.references | Sztein, J. (2013). Principios de criobiología. Genetic Engineering and Research Support. | |
dc.relation.references | Tabares, C. J., Tarazona, A. M., & Olivera, M. A. (2016). Fisiología de la activación del espermatozoide en peces de agua dulce. Revista Colombiana de Ciencias Pecuarias, 18(2), 13. https://doi.org/10.17533/udea.rccp.324003 | |
dc.relation.references | Torres, T. M., Almeida-Monteiro, P. S. D., Nascimento, R. V., Pereira, V. A., Ferreira, Y. M., Lobato, J. S., Pinheiro, R. R. R., Sales, Y. S., Montenegro, A. R., & Salmito-Vanderley, C. S. B. (2022). Sperm cryopreservation of Prochilodus brevis using different concentrations of non-permeable cryoprotectants. Animal Reproduction, 19(1). https://doi.org/10.1590/1984-3143-ar2021-0083 | |
dc.relation.references | Torres, T., de Almeida-Monteiro, P., do Nascimento, R., Cândido-Sobrinho, S., Sousa, C., Ferreira, Y., de Paula, K., & Salmito-Vanderley, C. (2024). Effects of taurine, cysteine and melatonin as antioxidant supplements to the freezing medium of Prochilodus brevis sperm. Cryobiology, 104, 858. https://doi.org/10.1016/j.cryobiol.2024.104858 | |
dc.relation.references | Vargas Licona, S. P., & Marrugo Negrete, J. L. (2019). Mercurio, metilmercurio y otros metales pesados en peces de Colombia: riesgo por ingesta. Acta Biológica Colombiana, 24(2), 232–242. https://doi.org/10.15446/abc.v24n2.74128 | |
dc.relation.references | Viveiros, A. T., & Godinho, H. P. (2009). Sperm quality and cryopreservation of Brazilian freshwater fish species: a review. Fish physiology and biochemistry, 35(1), 137–150. https://doi.org/10.1007/s10695-008-9240-3 | |
dc.relation.references | Warnecke, D., & Pluta, H.-J. (2003). Motility and fertilizing capacity of frozen/thawed common carp (Cyprinus carpio L.) sperm using dimethyl-acetamide as the main cryoprotectant. Aquaculture, 215(1–4), 167–185. https://doi.org/10.1016/S0044-8486(02)00371-X | |
dc.relation.references | Watson, P. F. (2000). The causes of reduced fertility with cryopreserved semen. Animal Reproduction Science, 60–61, 481–492. https://doi.org/10.1016/S0378-4320(00)00099-3 | |
dc.relation.references | Whaley, D., Damyar, K., Witek, R., Mendoza, A., Alexander, M., & Lakey, J. (2021). Cryopreservation: An overview of principles and cell-specific considerations. Cell Transplantation, 30, 963689721999617. https://doi.org/10.1177/0963689721999617 | |
dc.relation.references | Xin, M., Siddique, M., Dzyuba, B., Cuevas-Uribe, R., Shaliutina-Kolešová, A., & Linhart, O. (2017). Progress and challenges of fish sperm vitrification: A mini review. Theriogenology, 98, 16–22. https://doi.org/10.1016/j.theriogenology.2017.04.043 | |
dc.relation.references | Xin, N., Liu, T., Zhao, H., Wang, Z., Liu, J., Zhang, Q., & Qi, J. (2014). The impact of exogenous DNA on the structure of sperm of olive flounder (Paralichthys olivaceus). Animal Reproduction Science, 149, 305–310. https://doi.org/10.1016/j.anireprosci.2014.06.029 | |
dc.relation.references | Yang, H., Hu, E., Matthews, J. L., Varga, Z. M., & Tiersch, T. R. (2023). Is catalase an effective additive to alleviate oxidative stress during cryopreservation of zebrafish sperm at the repository level? Cryobiology, 104, 70–78. https://doi.org/10.1016/j.cryobiol.2021.10.003 | |
dc.relation.references | Yasui, G. S., Arias-Rodríguez, L., Fujimoto, T., & Arai, K. (2009). A sperm cryopreservation protocol for the loach Misgurnus anguillicaudatus and its applicability for other related species. Animal Reproduction Science, 116(3), 335–345. https://doi.org/10.1016/j.anireprosci.2009.02.021 | |
dc.relation.references | Zohar, Y., & Mylonas, C. C. (2001). Endocrine manipulations of spawning in cultured fish: From hormones to genes. Aquaculture, 197(1–4), 99–136. https://doi.org/10.1016/S0044-8486(01)00584-1 | |
dc.rights | Copyright Universidad de Córdoba, 2025 | |
dc.rights.accessrights | info:eu-repo/semantics/embargoedAccess | |
dc.rights.coar | http://purl.org/coar/access_right/c_f1cf | |
dc.rights.license | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | |
dc.subject.keywords | Sperm kinetics | eng |
dc.subject.keywords | Conservation | eng |
dc.subject.keywords | Osmolarity | eng |
dc.subject.keywords | Pimelodidae | eng |
dc.subject.proposal | Cinética espermática | spa |
dc.subject.proposal | Conservación | spa |
dc.subject.proposal | Osmolaridad | spa |
dc.subject.proposal | Pimelodidae | spa |
dc.title | Criopreservación de semen de bagre sapo Pseudopimelodus atricaudus | spa |
dc.type | Trabajo de grado - Maestría | |
dc.type.coar | http://purl.org/coar/resource_type/c_bdcc | |
dc.type.coarversion | http://purl.org/coar/version/c_ab4af688f83e57aa | |
dc.type.content | Text | |
dc.type.driver | info:eu-repo/semantics/masterThesis | |
dc.type.redcol | http://purl.org/redcol/resource_type/TM | |
dc.type.version | info:eu-repo/semantics/acceptedVersion | |
dspace.entity.type | Publication |
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