Publicación:
Cultivo de tejidos vegetales

dc.contributor.authorSuárez Padrón, Isidro Elíasspa
dc.contributor.graphicaldesignerGRÁFICAS DEL CARIBE S.A.Sspa
dc.date.accessioned2020-03-13T17:55:36Zspa
dc.date.available2020-03-13T17:55:36Zspa
dc.date.issued2020spa
dc.description.abstractEl cultivo de tejidos vegetales es una técnica biotecnológica que comprende el mantenimiento de plantas o componentes de estas en condiciones ambientales controladas, ausencia de microorganismos asociados, nutrición muchos aspectos del desarrollo agrícola y la investigación vegetal.spa
dc.description.tableofcontentsPRESENTACIÓN 11spa
dc.description.tableofcontents1. PREHISTORIA E HISTORIA DEL CULTIVO DE TEJIDOS VEGETALES 13spa
dc.description.tableofcontents1.1 El primer cultivo de tejidos 14spa
dc.description.tableofcontents1.2 La teoría celular y totipotencia 14spa
dc.description.tableofcontents1.3 El primer cultivo in vitro 14spa
dc.description.tableofcontents1.4 Crecimiento de tejidos in vitro 15spa
dc.description.tableofcontents1.5 Cultivo indefinido 16spa
dc.description.tableofcontents1.6 Micropropagación 17spa
dc.description.tableofcontents1.7 Cultivo indefinido de callo 17spa
dc.description.tableofcontents1.8 Cultivo de meristemos y Agrobacterium tumefaciens 17spa
dc.description.tableofcontents1.9 Citocininas y organogénesis 18spa
dc.description.tableofcontents1.10 Medios de cultivo 18spa
dc.description.tableofcontents1.11 Embriogénesis somática 19spa
dc.description.tableofcontents1.12 Se prueba la teoría celular 19spa
dc.description.tableofcontents2. EL LABORATORIO DE CULTIVO DE TEJIDOS VEGETALES 21spa
dc.description.tableofcontents2.1 Áreas del laboratorio 22spa
dc.description.tableofcontents2.2 Medidas de seguridad 26spa
dc.description.tableofcontents2.3 Equipos, utensilios y reactivos básicos necesarios 27spa
dc.description.tableofcontents3. TÉCNICA ESTÉRIL DE CULTIVO DE TEJIDOS VEGETALES 29spa
dc.description.tableofcontents3.1 Tipos de contaminantes en el cultivo in vitro 30spa
dc.description.tableofcontents3.2 Fuentes de contaminación 32spa
dc.description.tableofcontents3.3 Técnica estéril 34spa
dc.description.tableofcontents4. MEDIOS DE CULTIVO 41spa
dc.description.tableofcontents4.1 Componentes de un medio 42spa
dc.description.tableofcontents4.2 Formulación y preparación de medios de cultivo 50spa
dc.description.tableofcontents5. MICROPROPAGACIÓN DE PLANTAS 57spa
dc.description.tableofcontents5.1 Generalidades de la micropropagación 58spa
dc.description.tableofcontents5.2 Métodos de micropropagación de plantas 59spa
dc.description.tableofcontents6. SUSPENSIONES CELULARES 79spa
dc.description.tableofcontents6.1 Material vegetal 80spa
dc.description.tableofcontents6.2 Fases de un cultivo en suspensión celular 80spa
dc.description.tableofcontents6.3 Medición del crecimiento en suspensiones celulares 84spa
dc.description.tableofcontents7. CONSERVACIÓN DE GERMOPLASMA IN VITRO 87spa
dc.description.tableofcontents7.1 Almacenamiento por crecimiento lento 89spa
dc.description.tableofcontents7.2 Crioconservación 89spa
dc.description.tableofcontents8. CULTIVO DE PROTOPLASTOS 95spa
dc.description.tableofcontents8.1 Aislamiento de los protoplastos 96spa
dc.description.tableofcontents8.2 Aplicaciones del aislamiento y cultivo de protoplastos 99spa
dc.description.tableofcontents9. SELECCIÓN IN VITRO 101spa
dc.description.tableofcontents9.1 Selección in vitro de variantes somaclonales 102spa
dc.description.tableofcontentsBIBLIOGRAFÍA 105spa
dc.format.mimetypeapplication/pdfspa
dc.identifier.citationAcosta C. 2011. Propagación in vitro de árboles superiores de melina (Gmelina Robx.). Tesis M. Sc. Universidad de Córdoba, Montería.spa
dc.identifier.citationAcosta C, Suárez I y Gatti K. 2013.In vitro multiplication of Roxb. Adult trees. 16(1):97-102spa
dc.identifier.citationAgrios G. 2005. Plant Pathology. Elsevier, New York, p618-620, p689-690.spa
dc.identifier.citationAleza P, Garcia-Lor A, Juárez J y Navarro L. 2016. Recovery of citrus cybrid plants with diverse mitochondrial and chloroplastic genome combinations by protoplast fusion followed by in vitro shoot, root, or embryo micrografting.spa
dc.identifier.citationArditi J. 2008. Micropropagation of Orchids. Blackwell Publishing, Malden, p10-11.spa
dc.identifier.citationen segmentos nodales de roble (Tabebuia rosea), cedro (Cedrela odorata) y abarco (Cariniana pyriformis) a nivel in vitro. Tesis Ingeniero Agrónomo, Universidad de Córdoba, Montería.spa
dc.identifier.citationBairu M, Aremu A y Van Staden J. 2011. Somaclonal variation in plants: causes and detection methods. Plant Growth Regulation 63(2):147-173.spa
dc.identifier.citationBall E. 1946. Development in sterile culture of stem tips and subjacent regions of Tropaeolum majus L. and of Lupinus albus L. American Journal of Botany 33:301-318.spa
dc.identifier.citationBegheyn R, Roulund N, Vangsgaard K, Kopecký D y Studer B. 2017. Inheritance patterns of the response to in vitro doubled haploid induction in perennial ryegrass (Lolium perenne L.). Plant Cell, Tissue and Organ Culture 130: 667-679.spa
dc.identifier.citationBewley J y Black M. 1994. Seeds Physiology of Development and Germination.spa
dc.identifier.citationBroetjes C y Keen A. 1980. Adventitious shoots: Do they develop from one cell?. Euphytica 29:13-87.spa
dc.identifier.citationCaplin S y Steward F. 1948. Effect of coconut milk on the growth explants from carrot root. Science 108:655.spa
dc.identifier.citationCarlile M, Watkinson S y Gooday G. 2001. The Fungi. Academic Press, Londres, 565p.spa
dc.identifier.citationCarloni E, Tommasino E, López C, Ribotta A, Quiroga M, Griffa S y Grumberg K. 2017. In vitro selection and characterization of buffelgrass somaclones with different responses to water stress. Plant Cell, Tissue and Organ Culture 130: 265-277.spa
dc.identifier.citationCarmona O. 2017. Microtuberización en la especie Dioscorea rotundata cultivar botón. Tesis M. Sc. Biotecnología, Universidad de Córdoba, 70p.spa
dc.identifier.citationCassells A. 1991. Problems in tissue culture contaminants. En: Debergh P y Zimmermann R (Ed) Micropropagation: Technology and Application. Kluwer Academic, Dordrecht, p31-45.spa
dc.identifier.citationChawla H. 003. Introduction to Plant Biotechnology. Science Publisher, Enfield, p1-134, 87-109.spa
dc.identifier.citationChristianson M y Warnik D. 1988. Organogénesis in vitro as a developmental process. HortScience 23:215.spa
dc.identifier.citationChristianson M y Warnik D. 1983. Competence and determination in the process of in vitro shoot organogenesis. Developmental Biology 95:288-293.spa
dc.identifier.citationChristianson M y Warnik D. 1985. Temporal requirement for phytohormone balance in the control of organogenesis in vitro. Developmental Biology 112:494.spa
dc.identifier.citationDavies P. The plant hormones: Their nature, occurrence and function. En: Davis J. (ED) Plant Hormones, Biosynthesis, Signal Transduction, Action. Springer, Dordrecht, p1-15.spa
dc.identifier.citationDebergh P y Maene L. 1981. A scheme for commercial propagation of ornamental plants by tissue culture. Scentia Horticulturae 14:335-345.spa
dc.identifier.citationDíaz L. 2017. Micropropagación de ñame espino (Dioscorea rotundata Poir.) cultivar Botón en sistema de cultivo doble fase. Tesis M. Sc. Biotecnología, Universidad de Córdoba, Departamento de Química, Montería.spa
dc.identifier.citationDodeman V, Ducreux G y Kreis M. 1997. Zygotic embryogenesis versus somatic embryogenesis. 48(313):1493-1509.spa
dc.identifier.citationEfendi D y Litz R. 2003. Cryopreservation of avocado. Proceedings V World Avocado Congress, Valencia, España.spa
dc.identifier.citationEfendi D. 2003. Transformation and cryopreservation of embryogenic avocado (Persea americana Mill.) cultures. Tesis Ph.D., University of Florida, Gainesville.spa
dc.identifier.citationEngelmann F. 1998. In vitro germplasm conservation. Acta Horticulturae 461:41-48.spa
dc.identifier.citationFeher A, Pasternak T y Dudits D. 2003. Transition of somatic cells to an embryogenic state. Plant Cell, Tissue and Organ Culture 74:201-228.spa
dc.identifier.citationGamborg O y Shyluk J. 1981. Nutrition, media and characteristics of plant cell and tissue cultures. En: Thorpe T (Ed): Plant Tissue Culture Methods and Applications in Agriculture. Academic Press, New York, p21-44.spa
dc.identifier.citationGamborg O, Miller R y Ojima K. 1968. Plant cell cultures. I. Nutrient requirements of suspension cultures of soybean root cells. 50:151–158.spa
dc.identifier.citationGamborg O, Murashige T, Thorpe T y Vasil I. 1976. Plant tissue culture media. In Vitro 12(7):473-478.spa
dc.identifier.citationGao D, Vallejo V, He B, Gai Y y Sun L. 2009. Detection of DNA changes in somaclonal mutants of rice using SSR markers and transposon display. Plant Cell, Tissue and Organ Culture 98:187-196.spa
dc.identifier.citationGautheret R. 1939. Sur la possibilité de réaliser la culture indéfinie des tissus de tubercules de Carotte. Comptes Rendus de l´Academie des Sciences 208:118-130.spa
dc.identifier.citationGautheret R. 1985. History of plant tissue culture: A personal account. En: Vasil I (Ed) Cell Culture and Somatic Cell Genetics of Plants. Academic Press, New York, p18-35.spa
dc.identifier.citationGonzález-Benito M, Clavero-Ramírez I y López-Aranda J. 2004. Review. The use of cryopreservation for germplasm conservation of vegetatively propagated crops. Spanish Journal of Agricultural Research 2(3):341-351.spa
dc.identifier.citationGiri C y Praveena M. 2015. In vitro regeneration, somatic hybridization and genetic transformation studies: an appraisal on biotechnological interventions in grasses. Plant Cell, Tissue and Organ Culture 120: 843-860.spa
dc.identifier.citationGonzález F. 2012. Embriogénesis somática y organogénesis en caña flecha (Gynerium sagitatum Aubl.) Tesis M. Sc. Biotecnología, Universidad de Córdoba, Departamento de Química, Montería.spa
dc.identifier.citationGuevara Y, Suárez I y Salgado J. 2012. Inducción y proliferación in vitro de tejidos celulares de bata (Ipomoea batatas [L.] Lam.) en medio con 2,4-D. Temas Agrarios 17(2):9-17.spa
dc.identifier.citationGray D. 1996. Nonzygotic embryogenesis. En: Gray D y Trigiano R (Ed) Plant Tissue Culture Concepts and Laboratory Exercises. CRC Press, Boca Ratón, p133- 147.spa
dc.identifier.citationGrondeau C y Samson R. 1994. A review of thermotherapy to free plant materials from pathogens, specially seed from bacteria. Critical Review of Plant Science 13:57-75.spa
dc.identifier.citationGugerli P. 1992. Commercialization of serological tests for plant viruses. En: Duncan J y Torrance L (Ed) Techniques for the Rapid Detection of Plant Pathogens.spa
dc.identifier.citationHaberlandt G. 1902. Kulturversuche mit isolierten Pflanzenzellen. Sitz. Akad. Wiss. Wien 111:69-92.spa
dc.identifier.citationHaccius B. 1978. Question of unicellular origin of non-zygotic embryos in callus cultures. Phytomorphology 28(1):74-81.spa
dc.identifier.citationHalim N, Tan B, Midin R, Madon M y Khalid N. 2018. Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylasespa
dc.identifier.citationHalim N, Tan B, Midin R, Madon M y Khalid N. 2018. Abscisic acid and salinity stress induced somaclonal variation and increased histone deacetylase (HDAC) activity in Ananas comosus var. MD2. Plant Cell, Tissue and Organ Culture 133:123-35.spa
dc.identifier.citationHorts R y Klopmeyer M. 1993. Controlling viral diseases. En: White J (Ed) Geraniums IV. Ball Publishing, Geneve, p289-292.spa
dc.identifier.citationYayasankarS, Zhijian L y Gray D. 2000. In vitro selecction of Vitis vinifera “Chardonnay” with Elsinoe ampelina and enhanced secretion of chitinase. Planta 211:200-208.spa
dc.identifier.citationKane M. 1996. Micropropagation from pre-existing meristems, En: Gray D y Trigiano R (Ed) Plant Tissue Culture Concepts and Laboratory Exercises. CRC Press, Boca Ratón, p75-86.spa
dc.identifier.citationKlercker J. 1982. Eine methode zur isolierung lebender protoplasten. Ofvers Vetensk-Akad Förh Stokh 9:463-475.spa
dc.identifier.citationKlopmeyer M. 1996. Indexing for plant pathogens. En: Trigiano R y Gray D (Ed) Plant Tissue Cultures Concepts and Laboratory Exercises. CRC Press, Boca Raton, p341-349.spa
dc.identifier.citationKotte W. 1922. Kulturversuche mit isolierten Wurzelspitzen. Beitrage zur Allgemeinen Botanik 2: 413-434.spa
dc.identifier.citationKyte L y Kleyn J. 2003. Plants from Test Tubes. An Introduction to Micropropagation. Timber Press Portland, p90-100.spa
dc.identifier.citationLazar M, Chen T, Gusta L y Kartha K. 1998. Somaclonal variation for freezing tolerance in a population derived from norstar winter wheat . Theoretical and Applied Genetics 75:480-484.spa
dc.identifier.citationLitz R y Gray D. 1992. Organogénesis and somatic embryogenesis. En: Hamerschlag F y Litz R (Ed) Biotechnology of Perennial Fruit Crops. CAB International, Wallingford, p3-34.spa
dc.identifier.citationLitz R y Gray D. 1995. Somatic embryogenesis for agricultural improvement. World Journal of Microbiology and Biotechnology 11:416-425.spa
dc.identifier.citationLitz R, Raharjo S y Gómez-Lim M. 2007. Avocado. En: Nagata T, Lorz H y Widholm J (Ed) Biotechnology in Agriculture and Forestry. 60 Transgenic Crops V. Springer-Verlag, Berlin, p170-189.spa
dc.identifier.citationLloyd G y MaCown B. 1980 Commercially feasible micropropagation of mountain laural (Kalmia latifolia) by use of shoot tip culture. Combined Proceedings of International Societies 30:421-427.spa
dc.identifier.citationLópez C. 2017. Micropropagación de tres variedades de caña flecha (Gynerium sagitatum Aubl.). Tesis M. Sc. Biotecnologia, Universidad de Córdoba, Departamento de Química, Montería.spa
dc.identifier.citationLu S, Chen Ch, Wang Z, Guo Z y Li H. 2009. Physiological responses of somaclonal variants of triploid bermudagrass (Cynodon transvaalensis × Cynodon dactylon) to drought stress. Plant Cell Reports 28(3):517-526.spa
dc.identifier.citationMaheshwari P y Rangaswamy N. 1958. Polyembriony and in vitro culture of Citrus and Mangifera. Indian Journal of Horticulture 15:275-282.spa
dc.identifier.citationMalice M y Baudoin J. 2009. Genetic diversity and germplasm conservation of three minor Andean tuber crop species. Biotechnology, Agronomy, Society and Environment 13(3):441-448.spa
dc.identifier.citationMandal A, Pramanik S, Chowdhury B y Bandyopadhyay A. 1999. Salt-tolerant Pokkali somaclones: performance under normal and saline soils in Bay Islands. Field Crops Research 61(1):13-21.spa
dc.identifier.citationMarcotrigiano M y Gouin F. 1984. Experimentally synthesized plant chimeras 1. In vitro recovery of Nicotiana tabacum L. chimeras from mixed callus culture. Annals of Botany 54:503-511.spa
dc.identifier.citationMatthews R. 1981. Plant Virology. Academic Press, New York, 916p.spa
dc.identifier.citationMiller C, Skoog F, von Saltza M y Strong, M. 1995. Kinetin, a cell division factor from deoxyribonucleic acid. Journal of American Chemical Society 77:1329– 1334.spa
dc.identifier.citationMiller C. 1961. A kinetin-like compound in maize. Proceedings National Academy of Sciences 47:170-174.spa
dc.identifier.citationMorel G. 1963. La culture in vitro du méristème apical de certaines Orchidées. Comptes rendus hebdomadaires des séances de l’Académie des sciences 256:4955-4957.spa
dc.identifier.citationMurashige T y Skoog F. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.spa
dc.identifier.citationMurashige T. 1974. Plant propagation through tissue culture. Annual Review of Plant Physiology 25:135-166.spa
dc.identifier.citationNaik N, Rout P, Umakanta N, Verma R, Katara J, SahooK O y Samantaray S. 2017. Development of doubled haploids from an elite indica rice hybrid (BS6444G) using anther culture. Plant Cell, Tissue and Organ Culture 128:679-689.spa
dc.identifier.citationNitsch J y Nitsch C. 1969. Haploid plants from pollen grains. Science 163:85-87.spa
dc.identifier.citationNobecourt P. 1938. Sur les proliferations spontanées de fragments de tubercules de Carotte et leur culture sur milieu synthétique. Bulletin de la Société Botanique de France 85:1-7.spa
dc.identifier.citationNover L, Kranz E y Scharcf K. 1982. Growth cycle of suspension cultures of Lycopersicum sculentum and L. peruvianum. Biochemistry and Physiology 177:483-499.spa
dc.identifier.citationOgleeve-O´Donovan W. 1993. Culture indexing for vascular wilts and viruses. En: White J (Ed) Geraniums IV. Ball Publishing, Geneve, p227-286.spa
dc.identifier.citationPastrana I y Suárez I. 2009. Producción de plantas de caña flecha (Gynerium sagitatum Aubl.) “Criolla” a través de micropropagación. Temas Agrarios 14:5-13.spa
dc.identifier.citationPetit A, Delhaye S, Tempe´ J y Morel G. 1970. Reserches sur les guanidines des tisues de crown gall. Mise en evidence dúne relation biochemique Ágrobacterium tumefaciens et les tumores quélles induisent. Physiology Vegetable 8:205–213.spa
dc.identifier.citationPolanco H. 2017. Inducción de embriones somáticos en la especie Dioscorea rotundata Poir. Cultivares alemán y Botón. Tesis M. Sc. Biotecnología, Departamento de Química, Montería.spa
dc.identifier.citationPolo J. 2011. Micropropagación de teca (Tectona grandis) a partir de meristemos pre-existentes. Tesis M. Sc. Universidad de Córdoba, Montería.spa
dc.identifier.citationPolo J, Suárez I y Gatti K. 2013. Micropropagación de Tectona grandis L. F. a partir de meristemos pre-existentes. Temas Agrarios 18(2):83-93spa
dc.identifier.citationQuintero I, Polo J, Jarma A y Espitia A. 2003. Enraizamiento in vitro de Dioscorea sp. Revista Colombiana de Biotecnología 5(2):41-51.spa
dc.identifier.citationRaharjo S y Litz R. 2008. Recovery of avocado (Persea americana Mill.) plants transformed with the antifungal plant defensin gene PDF1.2. In Vitro and Cellular Development Biology-Plant 44:254-262.spa
dc.identifier.citationRahmani M, Pijut P y Shabanian, N. 2016. Protoplast isolation and genetically true-to-type plant regeneration from leaf- and callus-derived protoplasts of Albizia julibrissin. Plant Cell, Tissue and Organ Culture 127:475-488.spa
dc.identifier.citationRobbins W. I922. Cultivation of excised root-tips and stem tips under sterile conditions. Botanical Gazette 73: 376-390.spa
dc.identifier.citationRoca W, Arias D y Chávez R. 1993. Métodos de conservación in vitro de germoplasma En: Roca W y Mroginski L (Ed) Cultivo de Tejidos en la Agricultura, Fundamentos y Aplicaciones. CIAT, Cali, 697-714.spa
dc.identifier.citationSalgado J. 2011. Estudio in vitro de tejidos embriogénicos de ñame (Dioscorea alata Colletotrichum gloeosporioides. Tesis M. Sc. Biotecnología, Universidad de Córdoba, Montería.spa
dc.identifier.citationSalgado J y Suárez I. 2012. Respuesta de tejidos embriogénicos de ñame (Dioscórea alata Var Pico Botella) A Colletotrichum gloeosporioides. Revista Temas Agrarios 17(1):9-19spa
dc.identifier.citationSalgado J y Suárez I. 2012. Respuesta de tejidos embriogénicos de ñame (Dioscórea alata Var Pico Botella) A Colletotrichum gloeosporioides. Revista Temas Agrarios 17(1):9-19spa
dc.identifier.citationSauerweine M, Yoshimatsu K y Shimomoura K. 1992. Further approaches in the production of secondary metabolites by plant tissue culture. Plant Tissue Cultures Letters 9(1):1-9.spa
dc.identifier.citationSchleiden M. 1838. Beitrage zur Phytogenesis. Archiv für Anatomie, Physiologie und Wissenschaftliche Medicin:137-176.spa
dc.identifier.citationSchnell R, KunhnD, Olano C y QuintanillaW. 2001. Sequence dic¿versity among avocado sunblotch viroids isolated from single avocado trees. Phytoparasitica 29: 451–460.spa
dc.identifier.citationSchwann T. 1839. Mikroskopische Untersuchungen fiber die tJbereinstimmung in der Struktur und dem Wachstum der Thiere und Pflanzen. Nr. 170: Oswalds Berlin.spa
dc.identifier.citationSchwarz O y Beaty R. 1996. Propagation from nonmeristematic tissues- organogenesis. En: Gray D y Trigiano R (Ed) Plant Tissue Culture Concepts and Laboratory Exercises. CRC Press, Boca Ratón, p95-104.spa
dc.identifier.citationShen X, Chen J, Kane M y Henny R. 2007. Assessment of somaclonal variation in Dieffenbachia plants regenerated through indirect shoot organogenesis. Plant Cell, Tissue and Organ Culture 91:21-27.spa
dc.identifier.citationSingha S, Bissonette G y Double M. 1987. Methods for sterilizing instruments contaminated with Bacillus sp. from plant tissue cultures. HortScience 22:659.spa
dc.identifier.citationSingha S. 1984. Influence of twocommercial agars on in vitro shoot proloferation of Álmey´crabapple and ´Seckel´pear. HortScience 19(2):227-228.spa
dc.identifier.citationSkoog F y Miller C. 1957. Chemical regulation of growth and organ formation in plant tissues cultured in vitro. 11. The Biological Action of Growth Substances 118-131.spa
dc.identifier.citationSkoog F y Tsui C. 1948. Chemical control of growth and bud formation in tobacco stem segments and callus cultured in vitro. American Journal of Botany 35:782–787.spa
dc.identifier.citationStevenson F. 1956. The behavior of Citrus tissues and embryos in vitro. Ph. D. Dissertation, University of Michigan, Ann Arbor.spa
dc.identifier.citationSteward F, Mapes M y Mears K. 1958. Growth and development of cultured cells 2. Organization in cultures grown from freely suspended cells. American Journal of Botany 45:702-708.spa
dc.identifier.citationStreet H. 1977. Applications of cell suspension cultures. En: Reinert J y Bajaj P (Ed) Applied and Fundamentals Aspects of Plant Cell, Tissue and Organ Culture. Springer-Verlag, Berlin, p649-667.spa
dc.identifier.citationSuárez I. 2003. Avocado sunblotch viroid (ASBvd) in cell and tissue cultures of avocado (Persea americana Mill.). Tesis Ph. D., University of Florida, Gainesville.spa
dc.identifier.citationSuárez I y Jaraba J. 2004. Viroides: El otro tipo de fitpatógenos. Revista UDCA Actualidady Divulgación Cientifica 7(2)33-41.spa
dc.identifier.citationSuárez I, Litz R y Jaraba J. 2004. Embriogénesis somática en tres cultivares de aguacate (Persea americana Mill.). Temas Agrarios 9(2):32-41.spa
dc.identifier.citationSuárez I, Schnell R, Khun D y Litz R. 2005. Micrografting of ASBVd-infected avocado plants. Plant Cell, Tissue and Organ Culture 80:179-185.spa
dc.identifier.citationSuárezi, Espitia My Pertuz i. 2006. Efecto de auxinas y citocininas en la multiplicación y enraizamiento in vitro de Stevia rebaudiana Bertoni. Fitotecnia Colombiana 6(2):1-8.spa
dc.identifier.citationSuárez I, Jarma A y Avila M. 2006. Desarrollo de un protocolo para propagación in vitro de roble (Tabebuia rosea). Temas Agrarios 11(2):46-52.spa
dc.identifier.citationSuárez I, Schnell R, Khun D y Litz R. 2006. Recovery and indexing of avocado plants (Persea americana) from embryogenic nucellar cultures of an avocado sunblotch viroid-infected tree. Plant Cell, Tissue and Organ Culture 84:27-37.spa
dc.identifier.citationSuárez I y Salgado J. 2008. Propagación in vitro de Stevia rebaudiana Bert. (Asteraceae-Eupatoriae) a través de organogénesis. Temas Agrarios 13(1):33-40.spa
dc.identifier.citationSuárez I. 2008. Caracterizacion molecular, propagacion y conservacion in vitro de Gynerium sagitatum Aubl.) del caribe colombiano. División de Investigación, Universidad de Córdoba, Montería, 45p.spa
dc.identifier.citationSuárez I, Araméndiz H y Pastrana I. 2009. Micropropagación de caña flecha (Gynerium sagitatum Aubl.). Revista Facultad Nacional de Agronomía - Medellín 62(2):5135-5143.spa
dc.identifier.citationSuárez I y Quintero I. 2011. Micropropagación de Stevia rebaudiana Bertoni, un endulzante natural. VIII Congreso Internacional de Biotecnología Vegetal, Ciego de Ávila, Cuba.spa
dc.identifier.citationSuárez I, Torres L y Litz R. 2011. Somatic embryogenesis in yam (Dioscorea rotundata). Revista Facultad Nacional de Agronomía – Medellín 64(2):6037- 6042.spa
dc.identifier.citationSuárez I. 2011. Reguladores de Crecimiento Vegetal en el Cultivo de Tejidos In Vitro. XLI Congreso Anual COMALFI, Ibagué21-23 septiembre, p91-94.spa
dc.identifier.citationSuárez I, Rivera H y Pastrana I. 2012. Biotecnología aplicada ala caña flecha (Gynerium sagitatum Aubl.) . Fondo Editorial Universidad de Córdoba, Montería.spa
dc.identifier.citationSuárez I y Quintero I. 2014. Micropropagación de Stevia rebaudiana Bertoni, un endulzante natural, a través de explantes con meristemos pre-existentes. Revista Colombiana de Biotecnología 16(1):29-33.spa
dc.identifier.citationSuárez I y Otero R. 2016. Ácido abscísico y sacarosa afectan la producción in vitro de túberos de ñame (Dioscorea rotundata Poir.). Temas Agrarios 21(1):9-17spa
dc.identifier.citationSuárez I, Ortiz O y López C. 2017. Arrow cane (Gynerium sagitatum Aubl.) in vitro rhizome formation and plantlet recovery. Temas Agrarios 22(1):9-18spa
dc.identifier.citationSzabados L, Mroginski L y Roca W. 1993. Suspensiones celulares: descripción, manipulación y aplicación. En: Roca W y Mroginski L (Ed) Cultivo de Tejidos en la Agricultura, Fundamentos y Aplicaciones. CIAT, Cali, p173-210.spa
dc.identifier.citationSzabados L y Roca W. 1986. Plant regeneration from isolated mesophyll and cell suspension protoplasts of Stylosanthes guianensis. Plant Cell Reports 5: 174177spa
dc.identifier.citationTorres L. 2011. Micropropagación de acacia (Acacia mangium Willd.). Tesis M. Sc. Biotecnología, Universidad de Córdoba, Montería.spa
dc.identifier.citationTorres L, Suárez I y Gatti K. 3013. Propagación in vitro de Acacia mangium Willd. Biotecnología en el Sector Agropecuario y Agroindustrial 11(1):78-85spa
dc.identifier.citationUs-Camas R, Rivera-Solís G, Duarte-Aké F y De La Peña C. 2014. In vitro culture: an epigenetic challenge for plants. Plant Cell, Tissue and Organ Culture 118:187-201.spa
dc.identifier.citationVarghese D, Berjak P y Pammenter N. 2008. Alternate explants for germplasm cryopreservation of recalcitrant-seeded species: Problems and perspectives. South African Journal of Botany 74(2):391-392.spa
dc.identifier.citationVasil I y Hildebrandt C. 1965. Differentiation of tobacco plants from single isolated cells in microcultures. Science 150:889-890.spa
dc.identifier.citationVillalobos V y Thorpe T. 1993. Micropropagación: conceptos, metodología y resultados, En: Roca W Mroginski L (Ed) Cultivo de Tejidos en la Agricultura, Fundamentos y Aplicaciones. CIAT, Cali, p127-142.spa
dc.identifier.citationWhite P. 1934. Potentially unlimited growth of excised tomato root-tips in a liquid medium. Plant Physiology 9:585-600.spa
dc.identifier.citationWhite P. 1939. Potentially unlimited growth of excised plant callus in an artificial medium. American Journal of Botany 26: 59-64.spa
dc.identifier.citationWickson M y Thimann K. 1958. The antagonism of auxin and kinetin in apical dominance. Physiologia Plantarum 11:62-74.spa
dc.identifier.citationWitjaksono y Litz R. 1999a. Induction and growth characteristics of embryogenic avocado cultures. Plant Cell, Tissue and Organ Culture 59:19–29.spa
dc.identifier.citationWitjaksono, Litz R. 1999b. Maturation of avocado somatic embryos and plant recovery. Plant Cell, Tissue and Organ Culture 58:141–148.spa
dc.identifier.citationZhao F, Li Y, Hu Y, Zang X, Ding Q, Wang Y y Wen Y. 2016. A highly efficient grapevine mesophyll protoplast system for transient gene expression and the study of disease resistance proteins. Plant Cell, Tissue and Organ Culture 125:43-57spa
dc.identifier.citationZimmerman L. 1993. Somatic embryogenesis: a model for early development in higher plants. Plant Cell 5:1411-1423.spa
dc.identifier.isbn978-958-5104-09-9spa
dc.identifier.urihttps://repositorio.unicordoba.edu.co/handle/ucordoba/2553spa
dc.language.isospaspa
dc.publisherEd. Fondo Editorial Universidad de Córdobaspa
dc.publisher.facultyFacultad de Ciencias Agrícolasspa
dc.rightsCopyright Universidad de Córdoba, 2020spa
dc.rights.accessrightsinfo:eu-repo/semantics/restrictedAccessspa
dc.rights.creativecommonsAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)spa
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0/spa
dc.subject.keywordsculturespa
dc.subject.keywordswovenspa
dc.subject.keywordsVegetablesspa
dc.subject.proposalCultivospa
dc.subject.proposalTejidosspa
dc.subject.proposalVegetalesspa
dc.titleCultivo de tejidos vegetalesspa
dc.typeLibrospa
dc.type.coarhttp://purl.org/coar/resource_type/c_2f33spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bookspa
dc.type.redcolhttps://purl.org/redcol/resource_type/LIBspa
dc.type.versioninfo:eu-repo/semantics/submittedVersionspa
dspace.entity.typePublication
oaire.accessrightshttp://purl.org/coar/access_right/c_16ecspa
oaire.versionhttp://purl.org/coar/version/c_71e4c1898caa6e32spa
Archivos
Bloque original
Mostrando 1 - 1 de 1
Cargando...
Miniatura
Nombre:
Libro Cultivo de Tejidos Vegetales Edición 03-03-2020.pdf
Tamaño:
2.61 MB
Formato:
Adobe Portable Document Format
Descripción:
Bloque de licencias
Mostrando 1 - 1 de 1
No hay miniatura disponible
Nombre:
license.txt
Tamaño:
14.48 KB
Formato:
Item-specific license agreed upon to submission
Descripción:
Colecciones