Publicación: Efecto de la GnRh en protocolos de sincronización sobre la fertilidad de novillas Bos taurus indicus
dc.contributor.advisor | Salgado Otero, Roger Daniel | |
dc.contributor.author | Ortega Barrios, Laura Vanessa | |
dc.date.accessioned | 2023-06-26T18:39:49Z | |
dc.date.available | 2023-06-26T18:39:49Z | |
dc.date.issued | 2023-06-24 | |
dc.description.abstract | Hormonal treatments that combine the use of Gonadotropin Releasing Hormone (GnRH) and Prostaglandin F2α (PGF2α) have been able to synchronize estrus and ovulation in dairy cows and buffaloes, but in heifers the results have been very variable, reporting dispersion in estrus, persistence of follicles and low ovulation rates1. Therefore, in order to reduce the variability of these results, protocols have been created in combination with progesterone release devices; in this regard, the P4-Cosynch treatment that combines the use of Progesterone (P4) with GnRH and PGF2α, which has given good results in the synchronization of cows and heifers of dairy and beef breeds, stands out. According to ASOCEBÚ data, 95% of the Colombian cattle population is zebu or has zebu genetics. Therefore, this study could contribute to the dynamization of productive processes and to the increase of the breeding stock. Considering the above, the objective of this study was to evaluate the effect of GnRH hormone in fixed-term artificial insemination protocols on the fertility of Bos taurus indicus heifers. For the study, 158 clinically healthy Brahman cyclic and acyclic heifers with a body condition score of four were synchronized. The experimental treatments corresponded to six protocols that included the following hormones GnRH-PGF2-GnRH (T1 and T2), DIBGnRH-PGF2-GnRH (T3 and T4) and DIB-GnRH-PGF2-eCG-GnRH (T5 and T6). The animals were assessed for preovulatory follicle size by ultrasonography using an ALOKA Co., Ltd. UST-5820-5 equipped with a 5MHz linear translator, the screen was split in 2B mode, the image was frozen with the Freeze option, and the variables were analyzed by Chi-square test of independence and comparison of proportions between two populations. With the synchronization protocols, a total pregnancy rate of 10.7% (T1), 16.6% (T2) 36.8% (T3) 41.9% (T4) 38.7% (T5) and 27.68% (T6) was obtained. The effect (P= 0.0803) between treatments was found when comparing pregnancy proportions, it was found that T1 and T2 were statistically equal (p>0.05). The same happened with treatments T3, T4, T5 and T6, but differences (p≤0.05) were found between treatments T1 and T2 when compared with treatments T3, T4, T5 and T6. On the other hand, there were no differences (P ≥ 0.05), in the average preovulatory follicle size being these 10.28; 11.21 and 11.77 mm for heifers synchronized with treatments 1-2, 3-4 and 5-6, respectively. The use of GnRH hormone in combination with progesterone in Cosynch protocol significantly improves pregnancy rates in Bos indicus heifers, compared to the traditional Cosynch protocol. | eng |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Médico(a) Veterinario(a) y Zootecnia | spa |
dc.description.modality | Trabajos de Investigación y/o Extensión | spa |
dc.description.resumen | Los tratamientos hormonales que combinan el uso de la Hormona liberadora de Gonadotropina (GnRH) y Prostaglandina F2α (PGF2α) han logrado sincronizar el celo y la ovulación en vacas lecheras y búfalas, pero en novillas los resultados han sido muy variables, reportándose dispersiones en celo, persistencia de folículos y bajas tasas de ovulación1 . Por lo tanto, y en aras de disminuir la variabilidad de estos resultados se han creado protocolos en combinación con dispositivos de liberación de progesterona; al respecto se resalta el tratamiento P4-Cosynch que combina el uso de Progesterona (P4) con GnRH y PGF2α, el cual ha dado buenos resultados en la sincronización de vacas y novillas de razas lecheras y cárnicas. Puesto que según datos de ASOCEBÚ, el 95% de la población bovina colombiana es cebú o tiene genética cebuina. Por lo cual este estudio podría contribuir en la dinamización de procesos productivos y al aumento del pie de cría. Teniendo en cuenta lo anterior, el objetivo de este estudio fue evaluar el efecto de la hormona GnRH en protocolos de inseminación artificial a término fijo sobre la fertilidad de novillas Bos taurus indicus. Para el estudio se sincronizaron 158 novillas cíclicas y acíclicas de la raza Brahman clínicamente sanas y con un puntaje de condición corporal de cuatro. Los tratamientos experimentales correspondieron a seis protocolos que incluyeron las siguientes hormonas GnRH-PGF2-GnRH (T1 y T2), DIB-GnRH-PGF2-GnRH (T3 y T4) y DIB-GnRHPGF2-eCG-GnRH (T5 y T6). A los animales se les evaluó el tamaño del folículo preovulatorio por medio de ultrasonografía utilizando un ecógrafo ALOKA Co., Ltda. UST5820-5 equipado con un traductor lineal de 5MHz, la pantalla fue dividida en modo 2B, la imagen fue congelada con la opción Freeze, y las variables fueron analizadas mediante prueba de independencia de Chí cuadrado y comparación de proporciones entre dos poblaciones. Con los protocolos de sincronización se obtuvo una tasa de preñez total de 10,7 %(T1), 16,6% (T2) 36,8% (T3) 41,9 %(T4) 38,7% (T5) y 27,68% (T6). Se encontró efecto (P= 0.0803) entre los tratamientos al realizar la comparación de proporciones de preñez, se encontró que el T1 y T2 fueron estadísticamente iguales (p>0.05). Igualmente sucedió con los tratamientos T3, T4, T5 y T6, pero se encontró diferencias (p≤0.05) entre los tratamientos T1 y T2 al compararlos con los tratamientos T3, T4, T5 y T6. Por su parte, no hubo diferencias (P ≥ 0,05), en el promedio del tamaño de folículo preovulatorio siendo éstos 10,28; 11,21 y 11,77 mm para las novillas sincronizadas con los tratamientos 1-2, 3-4 y 5-6, respectivamente. El uso de la hormona GnRH en combinación con progesterona en protocolo Cosynch mejora significativamente las tasas de preñez en novillas Bos indicus, en comparación al protocolo Cosynch tradicional. | spa |
dc.description.tableofcontents | INTRODUCCIÓN 1 | spa |
dc.description.tableofcontents | 1. OBJETIVOS 3 | spa |
dc.description.tableofcontents | 1.1 OBJETIVO GENERAL 3 | spa |
dc.description.tableofcontents | 1.2 OBJETIVOS ESPECÍFICOS 3 | spa |
dc.description.tableofcontents | 2. MARCO TEÓRICO 4 | spa |
dc.description.tableofcontents | 2.1 PROTOCOLO COSYNCH 4 | spa |
dc.description.tableofcontents | 2.2 USO DE PROGESTERONA EN EL PROTOCOLO CO SYNCH (P4-COSYNCH) 4 | spa |
dc.description.tableofcontents | 2.3 USO DE LA ECG EN PROTOCOLOS DE IATF 5 | spa |
dc.description.tableofcontents | 3. MATERIALES Y MÉTODOS 7 | spa |
dc.description.tableofcontents | 3.1 TIPO DE ESTUDIO 7 | spa |
dc.description.tableofcontents | 3.2 LOCALIZACIÓN 7 | spa |
dc.description.tableofcontents | 3.3 POBLACIÓN Y MUESTRA 7 | spa |
dc.description.tableofcontents | 3.4 TECNICAS DE RECOLECCIÓN DE DATOS 7 | spa |
dc.description.tableofcontents | 3.4.1 Tratamiento Cosynch tradicional 8 | spa |
dc.description.tableofcontents | 3.4.2 Tratamiento Cosynch con progesterona 8 | spa |
dc.description.tableofcontents | 3.4.3 Tratamiento Cosynch con Progesterona y eCG 9 | spa |
dc.description.tableofcontents | 3.4.4 Ultrasonografía ovárica, respuesta ovulatoria y diagnóstico de preñez 9 | spa |
dc.description.tableofcontents | 3.5 ANALISIS ESTADÍSTICO 10 | spa |
dc.description.tableofcontents | 4. ASPECTOS ÉTICOS 11 | spa |
dc.description.tableofcontents | 5. RESULTADOS, DISCUCIÓN Y CONCLUSIONES 12 | spa |
dc.description.tableofcontents | BIBLIOGRAFÍA 19 | spa |
dc.format.mimetype | application/pdf | spa |
dc.identifier.uri | https://repositorio.unicordoba.edu.co/handle/ucordoba/7392 | |
dc.language.iso | spa | spa |
dc.publisher.faculty | Facultad de Medicina Veterinaria y Zootecnia | spa |
dc.publisher.place | Berástegui, Córdoba, Colombia | spa |
dc.publisher.program | Medicina Veterinaria y Zootecnia | spa |
dc.rights | Copyright Universidad de Córdoba, 2023 | spa |
dc.rights.accessrights | info:eu-repo/semantics/openAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0) | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0/ | spa |
dc.subject.keywords | Cosynch | eng |
dc.subject.keywords | Follicles | eng |
dc.subject.keywords | Hormones | eng |
dc.subject.keywords | IATF | eng |
dc.subject.keywords | Pregnancy rate | eng |
dc.subject.proposal | Cosynch | spa |
dc.subject.proposal | Folículos | spa |
dc.subject.proposal | Hormonas | spa |
dc.subject.proposal | IATF | spa |
dc.subject.proposal | Tasa de preñez | spa |
dc.title | Efecto de la GnRh en protocolos de sincronización sobre la fertilidad de novillas Bos taurus indicus | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.version | info:eu-repo/semantics/submittedVersion | spa |
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oaire.accessrights | http://purl.org/coar/access_right/c_abf2 | spa |
oaire.version | http://purl.org/coar/version/c_ab4af688f83e57aa | spa |
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