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    Diversidad genética en poblaciones humanas mediante polimorfismos de inserción Alu en el municipio de Moñitos- Córdoba
    (2020-11-13) Álvarez Villegas, Lizky Paola; Pardo Pérez, Enrique
    Alu insertion elements are DNA sequences that are produced by a single mutational event and their ancestral status is known, it is estimated that there are more than a million copies of these elements, that is, they occupy approximately 11% of the human genome. The objective of the present investigation was to determine the genetic diversity of the human population of the municipality of Moñitos-Córdoba, through the use of Alu insertion polymorphisms in 50 unrelated individuals from five selected populations in the municipality. The study was carried out from saliva samples, from which the DNA was extracted, later the DNA amplification was carried out by PCR and the amplified ones were observed in agarose gels, finally through statistical analysis the following were determined: allelic frequencies, Observed heterozygosities (Ho), Expected heterozygosities (He), Fixation index, Hardy-Weinberg equilibrium and Dendograms. All Alu markers were polymorphic, with frequencies ranging between 0.100 (A25) and 0.944 (HS3.23), the average values obtained for Ho and He were respectively 0.398 and 0.384, most of the markers were found in equilibrium of Hardy-Weinberg, except for the ACE and TRA25 markers, the genetic distance between the different populations studied, showed Miramar and Flores de Manga as the most related and Santa Lucia as the most distant among the populations evaluated. In conclusion, Moñitos population presented a high polymorphic level and low values of observed and expected heterozygosity, which indicates a low rate of genetic diversity.