G.B.A. Trabajos de Investigación y/o Extensión

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Examinando G.B.A. Trabajos de Investigación y/o Extensión por Materia "Alimento vivo"

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  • Miniatura
    PublicaciónAcceso abierto
    Crecimiento poblacional y fecundidad de Brachionus Ibericus alimentado con Microalgas
    (2021-01-25) Hernandez Ayala, Maria Claudia; Ruiz Martínez, Ricardo José; Prieto Guevara, Martha Janeth; Jiménez Velásquez, Cesar Augusto
    En el laboratorio de alimento vivo de la Universidad de Córdoba se evaluó el crecimiento poblacional, fecundidad y composición proximal del rotífero Brachionus ibericus con diferentes dietas microalgales en dos fases experimentales: En la fase I tres dietas algales Nannochloropsis oculata (T1); Isochrysis galbana (T2); Chaetoceros sp (T3) fueron evaluadas en concentración de 1x106cel.mL-1, con densidad inicial de 8 rot. mL-1. En la Fase II Isochrysis galbana fue evaluada en diferentes concentraciones 1x106 cel. mL-1 (T1), 3x106 cel. mL-1 (T2) y 6x106 cel. mL-1 (T3), con densidad inicial de 50 rot. mL-1. En la fase I y II, fueron usadas 12 unidades experimentales en vidrio con 2,5 litros de agua marina, con aireación constante y fotoperiodo 10-14 horas (luz: oscuridad). Diariamente se registró la temperatura (ºC), salinidad (ups), oxígeno disuelto (mg/L), y pH. Los parámetros poblacionales: densidad máxima (DM), tiempo de duplicación (TD); tasa instantánea de crecimiento (K); producción diaria (PD) fueron establecidas. La mayor densidad de organismos se presentó en T2 (107.33 ± 1.59 rot.mL-1) en fase I y con T3 en la fase II con los mejores resultados significativos (p>0.05) de DM (340.13 ± 20.83 rot/.mL-1), K (0.910 ± 0.004 días-1), TD (0.003 ± 0.006 días), PD (142,500 ± 1,345 rot/.mL-1.dia-1), fecundidad (1.94 ± 0.07 huevos.hembra-1) y composición proximal: Proteína MS (51.1 ± 7.0), Proteína MN (8.4 ± 1.1), Extracto Etéreo MS (31.9 ± 4.1) Extracto Etéreo MN (5.2 ± 0.7), Humedad (85.9 ± 0.1) y Ceniza (1.6 ± 0.04). El rotífero Brachionus ibericus muestra propiedades ideales para su cultivo intensivo y apropiada composición proximal con la microalga Isochrysis galbana, constituyéndose como una alternativa más para su uso como alimento vivo en la acuicultura.
  • Miniatura
    PublicaciónAcceso abierto
    Viabilidad de huevos acartia tonsa (Copepoda: calanoidea) de la bahía Cispatá – Caribe colombiano
    (2023-06-23) Hernández Casarrubia, Carmelo David; Jiménez Velásquez, César Augusto; Torres Valencia, Gustavo Adolfo; Prieto Guevara, Martha Janeth
    In the live food laboratory of the University of Córdoba, experiments were carried out to establish the effect on specific fecundity, hatching success and morphometric characteristics of the calanoid tropical copepod Acartia tonsa (Bahía de Cispatá) of T1 microalgae diets: Isochrysis galbana (IG), T2: Chaetoceros muelleri (CHA), and T3: the mixture of both algae (IG+CHA) in proportion (1:1) at a concentration of 1500 micrograms of carbon per liter (μgCL−1). likewise, the hatching of eggs spawned and stored at low temperature was evaluated. Experiment 1. The specific fecundity was determined in 225 adult copepods fed with the experimental diets, distributed in three chambers of 6 wells (5 mL/pz) for a total of 18 experimental units; during 5 days the eggs were harvested every 24 hours in each unit, with a record of Female Eggs-1day-1. Experiment 2. Egg hatching was determined in 80 to 100 eggs collected in each experimental diet and distributed in three chambers of 6 wells (10 mL/pz), 48 hours later in each unit the hatched nauplii were quantified under a microscope. Experiment 3. The morphometric characteristics of eggs, nauplii, females, and adult males of A. tonsa, were recorded under a microscope with a micrometric eyepiece in 20 copepods collected at different stages of development on each experimental diet. Experiment 4. The hatching of eggs spawned at five temperatures (18, 20, 23, 26 and 29 °C) and stored cold (2-4 0C) for 4 weeks, was determined weekly in adults housed in triplicate in containers of 2 L at 2.5 copepods.mL−1, fed with the microalga Isochrysis galbana at 1500 μgCL−1. Experiment 1. The highest fecundity was recorded on the third day for T1 (15.0 ± 7.0 eggs day-1 female-1) and T3 (14.0 ± 8.0 eggs female-1 day-1), followed by T2 (6.0±3.0 eggs day-1 female-1) on the first day. Experiment 2. The highest hatching percentage was recorded for T2 (78.8 ± 11.0%), followed by T1 (72.8 ± 7.4%) and T3 (69.2 ± 13.2%). Experiment 3. The diameter of the egg ranged between 85.7 ± 2.0 µm (T2) and 84.3 ± 2.2 µm (T1). The body length (LC) in nauplii ranged between 112.4 ± 8.52 µm (T2) and 115.2 ± 8.2 µm (T3). The females presented a total length (TL) between 997.5 ± 16.0 µm (T1) and 976.3 ± 27.5 µm (T3), and the males a TL between 850.0 ± 31.4 µm (T1) and 843.8±39.6 µm (T3). Experiment 4. At a temperature of 18 °C the highest hatching success was recorded for eggs stored in cold during the four weeks (87.3; 85.9; 83.0 and 73.2%). Microalgae diets for the copepod A. tonsa allowed adequate fecundity and hatching success of the eggs produced. Hatching of cold-stored resting eggs was affected by spawning temperature.