Examinando por Autor "Rodríguez, Virginia"

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    Determinación de la seroprevalencia de anticuerpos para brucella abortus en trabajadores de un frigorífico y ordeñadores en Montería, Córdoba (Colombia)
    (2016-11-15) Quintero, Gustavo; Calderón, Alfonso; Rodríguez, Virginia; Barrios, Cindy; Yasnot, María Fernanda; Villadiego, Marilyn
    Brucellosis is a bacterial zoonosis, considered an occupational disease that has high socioeconomic impact, with more than 500,000 new cases each year worldwide. In Colombia there are very few studies, temporally and geographically isolated. The population at risk are salaugtherhouse’s workers, milkers, veterinarians and bacteriologists. Traditionally, the diagnosis has been made by Rose Bengal and confirmed by competitive ELISA or pathogen isolation. The purpose of this study was the detection of antibodies to Brucella abortus in slaughterhouse’s workers and milkers in Monteria, Cordoba (Colombia) and, additionally, the two conventional methods were compared with Fluorescence Polarization. Blood samples were taken without anticoagulation from 162 workers and processed for antibodies against B. abortus with Rose Bengal, competitive ELISA and two Fluorescence Polarization protocols. A survey to determine the level of knowledge and attitudes that have volunteers about the problem was implemented. It was found that 7.41% of the samples were positive by Rose Bengal, 2.8% with protocol 1 and 15.33% with protocol 2 of fluorescence polarization. The samples that were positive with either method were confirmed by competitive ELISA. A seroprevalence of 0.7% was found. In this population there is a low seroprevalence of antibodies to B. abortus; 50.81% of the slaughterhouse workers and 47.11% of the farms workers, know the disease and assume attitudes to prevent contagion.
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    Meningitis bacterianas agudas en un centro hospitalario de Sincelejo, Sucre
    (Universidad de Córdoba, 2024-07-17) Chica Díaz, Jaime Andrés; Tique Salleg, Vaneza; Torres Arroyo, Ena Luz; Gracia Herrera, Lisy; Arrieta, Germán; Brango, Eimi; Rodríguez, Virginia; Ameth Salim, Mattar; Salim, Mattar
    Objetivo. Realizar una vigilancia de las meningitis bacterianas agudas (MBA) en un centro hospitalario de Sincelejo, Sucre. Materiales y métodos. Se realizó un estudio descriptivo prospectivo de corte transversal para identificar los casos de meningitis bacteriana aguda en un centro hospitalario de Sincelejo, Sucre. Se incluyeron pacientes hospitalizados con signos de meningitis bacteriana aguda entre agosto del 2021 a junio del 2023. Se uso qPCR para detectar S. pneumoniae, H. influenzae y N. meningitidis. Resultados. Se incluyeron 80 pacientes, se confirmó la MBA en el 16.3% (n=13) de los casos. De estos casos el 54% (n=7) correspondieron a pacientes pediátricos, y el 46% (n=6) a pacientes adultos. Los microorganismos principales identificados fueron: S. pneumoniae 30.7% (n=4), N. meningitidis 23.1% (n=3), y H. influenzae 7.7% (n=1). Otros gérmenes infrecuentes fueron aislados en cultivo (E. coli, Pseudomonas aeruginosa, Enterococcus gallinarum, Staphylococcus hominis, Staphylococcus arlettae). Conclusiones. El estudio permitió identificar los principales agentes etiológicos de las MBA en una clínica de Sincelejo. La qPCR permitió superar las limitaciones diagnósticas del cultivo en términos de tiempo, sensibilidad y oportunidad en las MBA.