Examinando por Autor "Martínez Bravo, Caty Milena"
Mostrando 1 - 3 de 3
Resultados por página
Opciones de ordenación
Publicación Acceso abierto Caracterización de la infección por SARS-CoV-2 en perros y gatos en 8 municipios del departamento de Córdoba, Colombia(SISTEMA GENERAL DE REGALIAS (SGR) Proyecto BPIN 20200000100090, 2022-08-25) Botero Serna, Yesica; Martínez Bravo, Caty Milena; Calderón Rangel, AlfonsoCOVID-19 is an emerging disease of possible zoonotic origin caused by the SARS-CoV-2 virus. It is presumed to have originated in an animal and passed from that animal to humans, in which it has spread very successfully since it was first discovered in late 2019. However, its return from humans to other animal species and again back to humans has been demonstrated, which has involved several mutations and variants that have affected the dynamics of SARS-CoV-2 infection. The possible routes of transmission and its zoonotic potential are currently under investigation worldwide; however, in Colombia, the epidemiological situation of this pathology cannot be accurately estimated due to the low notification of human and animal cases and fragmented epidemiological surveillance systems. Therefore, carrying out strategies to control zoonoses, such as emerging zoonotic disease surveillance, would benefit the region and the country with an awareness and understanding of the synergies involved under the "One Health" approach. Objective. To characterize SARS-CoV-2 infection in dogs and cats in 8 municipalities of the department of Córdoba, Colombia. Methodology. With the approval of the ethics committee of the Instituto de Investigaciones Biológicas del Trópico (IIBT) No. 0410-2020 and the ethics committee of the Faculty of Medicina Veterinaria y Zootecnia of the Universidad de Córdoba Acta No. 005 26 (May 2021). A cross-sectional descriptive molecular and serological surveillance was carried out, where oral and rectal swabs and blood samples were taken proportionally to the estimated population of dogs and cats in the 8 municipalities of the department using the following strategies: house-to-house sampling, deworming, and vaccination campaigns with departmental partners and veterinary centers, and direct request between September 2020 and September 2021. Results. Under the one health approach, this study provided the first genomic report of SARS-CoV-2 B.1.111 lineage infection in a cat in Colombia and the first human-dog transmission event of the IOTA variant in Latin America. Four active cases and an infection rate of 11.5% were identified. Three scientific articles were generated, two presented in a Q2 and a Q1 journal in English, and one to be submitted to a Q2 journal in Spanish where the data obtained from the present investigation were presented and made public. Conclusion. The characterization of SARS-CoV-2 infection in domestic animals living in the department of Córdoba provided relevant information to motivate further research by implementing interdisciplinary and different serological, molecular, and genomic methods of epidemiological surveillance.Publicación Acceso abierto Efecto de la fragmentación del paisaje sobre la diversidad de mamíferos terrestres en la vereda El Diamante, Tierralta - Córdoba(2020-11-10) Romero Oviedo, María José; Martínez Bravo, Caty MilenaFragmentation processes are mainly caused by changes in land use in natural ecosystems, which generates changes in the distribution, reproduction and survival of biodiversity. The objective of this study was to record the effect of landscape fragmentation on the diversity of terrestrial mammals in the village of El Diamante, Tierralta - Córdoba. The work was carried out in forest covers with 3 levels of fragmentation: high, medium and low. Data collection was done using the methodology proposed by Steenmans and Pinborg (1999), for the indicators of fragmentation and trapping in mammalian diversity. 119 records of land mammals were generated, belonging to 9 species, distributed in 5 orders and 8 families. The greatest diversity of terrestrial mammals was obtained in the low fragmentation covers.Publicación Acceso abierto Validación analítica de un kit de extracción in house para la amplificación de ácido ribonucleico de sars-cov-2(2023-07-13) Dueñas Argumedo, Yemima; Gastelbondo Pastrana, Bertha Irina; Martínez Bravo, Caty MilenaLa emergencia sanitaria más grave de los últimos tiempos, producida por el virus SARS-CoV-2 generó un colapso en la salud y la economía de casi todos los países del mundo y ocasionó un déficit de recursos para su detección. La repentina aparición de la enfermedad trajo consigo un arduo proceso investigativo para la invención y mejora de técnicas moleculares indispensables para la detección de este virus. En este sentido, investigadores del Instituto de Investigaciones Biológicas del Trópico IIBT de la Universidad de Córdoba desarrollaron un kit in house para la extracción de ARN de SARS-CoV-2 y otros agentes biológicos de alto riesgo. En el siguiente trabajo, se tuvo como objetivo validar un kit de extracción in house para la amplificación de ácido ribonucleico de SARS-CoV-2, en el cual se determinó la estabilidad del kit, se realizó extracción de ARN a 50 muestras de hisopados nasofaríngeos de pacientes con síntomas de COVID-19 procesando la misma muestra con un kit comercial por perlas magnéticas y el kit de extracción in house. Se cuantificó y determinó la pureza del ARN extraído con los distintos protocolos mediante un espectrofotómetro, se realizó una RT-qPCR para detectar Gen E (gen de la proteína E de SARS-CoV-2) y RNasa P (Gen utilizado como control interno) y los resultados de Cq obtenidos para cada kit fueron comparados. Se determinó un porcentaje de positividad para estos datos.