Examinando por Autor "Begambre Hernández, Mauricio"
Mostrando 1 - 2 de 2
Resultados por página
Opciones de ordenación
Publicación Acceso abierto Diversidad y estructura genética de la guayaba dulce (Psidium guajava l.) evaluada mediante marcadores microsatélites en tres municipios de Córdoba-Colombia(2022-07-25) Begambre Hernández, Mauricio; Pardo Pérez, EnriqueSweet guava (Psidium guajava L.) is a fruit tree of the Myrthaceae family with a wide distribution in tropical and subtropical areas, it is of great commercial interest due to its fruits with a pleasant flavor and high nutritional value. Due to its economic importance and its autochthonous condition in the department of Córdoba-Colombia, three populations of P. guajava corresponding to the municipalities of Sahagún, Tierralta and Cereté were characterized by microsatellite-type molecular markers (SSR). Of the twelve markers evaluated, seven were polymorphic and presented a total of 44 alleles distributed in three populations. The Polymorphic Information Content (PIC) was variable in the territories, finding the markers mPgCIR07 and mPgCIR20 as highly informative. The heterozygosity observed was 0.179, 0.081 and 0.555 for Sahagún, Tierralta and Cereté, respectively. The coefficient of differentiation between populations has an average of FST = 0.271 and a percentage of interpopulation variation of 7%, these results were reflected in the Bayesian clustering with DeltaK = 3 as the most probable number of genetic groups and in the differential distribution of the three populations in the principal coordinate analysis (PCoA). Cereté was characterized by a high genetic diversity as a result of the high family agricultural activity and productivity of this species in the soils of the present municipality, while the guava populations of the municipalities of Tierralta and Sahagún presented high inbreeding and a high interpopulation genetic differentiation was found, probably attributed to little interest in its production.Publicación Acceso abierto Evaluación de daños genotóxicos en eritrocitos de peces del género andinoacara capturados en el caño Berástegui con incidencia de aguas residuales provenientes de la ciudad de Montería-Córdoba(2020-11-12) Lugo Pérez, Yulis Patricia; Hernández López, Luis Ángel; Begambre Hernández, MauricioWith the objective of evaluating the influence of wastewater from the Berástegui channel on the formation of micronuclei and nuclear alterations (cells with blisters, lobes and slits) in erythrocytes of fish of the Andinoacara genus. 10 specimens were captured in four different sites of the channel (S1, S2, S3, S4) that were compared with a tributary without influence of wastewater that was taken as a negative control. From which blood samples were taken from a puncture in the gills, which were fixed and stained. The frequency of micronuclei and nuclear alterations was quantified from the count of 2,000 erythrocytes per individual. Significant differences were found in the micronuclei and nuclear alterations between all the sites, and the sampling stations near the city of Montería reported the highest frequencies of micronuclei and nuclear alterations, which occurred in a higher proportion than micronuclei. These results confirm that the discharge of wastewater generated by the city of Montería without the implementation of treatments, influences the formation of micronuclei and nuclear alterations in fish species and the eutrophication of aquatic ecosystems. This shows that the quantification of micronuclei and nuclear alterations in situ in fish of the genus Andinoacara can be used to evaluate the incidence of different xenobiotic pollutants in the alteration of genetic material.