Ricardo Caldera, Dina MarcelaAvilés Vergara, Paula Andrea2022-01-252022-01-252022-01-24https://repositorio.unicordoba.edu.co/handle/ucordoba/4773Objective To determine simultaneous circulation of DENV serotypes and ZIKV in Córdoba, Colombia, during 2015 and 2016. Material and methods A total of 294 samples from patients with clinical diagnosis of febrile syndrome compatible with dengue were collected between June 2015 and December 2016. All samples were tested for DENV and ZIKV by RT-PCR using C6/36 cells culture supernatant. Results Thirty-three percent of the samples were positive (97/294); from these, 61.8% were positive for DENV and 31% were positive for Zika. The predominant serotype was DENV-2 (70.1%), followed by DENV-3 (8.9%), DENV-4 (6%), and DENV-1 (3%). DENV/ZIKV coinfection was identified in 7.2% of the cases associated with DENV-2 and DENV-4 serotypes. The confirmed cases of dengue, Zika, and DENV/ZIKV coinfections were clinically mild and self-limited. Conclusions We reported the co-circulation of all four DENV serotypes, with a higher frequency of DENV-2. ZIKV introduction in Córdoba department-Colombia in August 2015 favored the appearance of DENV/ZIKV coinfections.1. INTRODUCCIÓN ..................................................................................................................................... 102.JUSTIFICACIÓN................................................................................................................................133.MARCOTEÓRICO.............................................................................................................................143.1Epidemiología.............................................................................................................................. 143.1.1 Serotipos y genotipos del DENV.............................................................................................. 153.2 Biología del virus ....................................................................................................................... 163.2.1 Estructura y genoma del DENV ............................................................................................... 163.2.2 Transmisión y ciclo de vida ..................................................................................................... 183.3 Interacciones primarias del DENV con componentes de la respuesta inmune innata. ............. 193.3.1 Respuesta inmunitaria innata contra el DENV........................................................................ 193.3.2 Respuesta inmunitaria adaptativa contra el DENV ................................................................. 203.4 Inmunopatogénesis de la enfermedad por el virus del dengue ................................................ 213.4.1 Mejora de la enfermedad dependiente de anticuerpos (ADE) ............................................... 213.4.2 El fenómeno del pecado antigénico original........................................................................... 223.5 Diagnóstico................................................................................................................................. 233.6 Vigilancia, prevención y control ............................................................................................... 244. OBJETIVOS................................................................................................................................... 254.1 Objetivo general ........................................................................................................................ 254.2 Objetivos específicos.................................................................................................................. 255. METODOLOGÍA ............................................................................................................................ 265.1 Tipo, lugar y diseño del estudio.................................................................................................. 265.2 Muestras..................................................................................................................................... 275.3 Aislamiento viral en línea celular C6/36..................................................................................... 275.4 Detección Molecular por RT-PCR para DENV ........................................................................... 285.5 RT-PCR ZIKV................................................................................................................................ 285.6 Análisis estadístico...................................................................................................................... 295.7 Consideraciones éticas .............................................................................................................. 296. RESULTADOS................................................................................................................................ 307. DISCUSIÓN.................................................................................................................................... 348. CONCLUSIONES ........................................................................................................................... 379. PERSPECTIVAS Y RECOMENDACIONES ........................................................................................ 3810. REFERENCIAS BIBLIOGRÁFICAS................................................................................................... 3911. ANEXOS....................................................................................................................................... 46application/pdfspaCopyright Universidad de Córdoba, 2022Trabajo de grado - Maestríainfo:eu-repo/semantics/openAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)Virus del dengueVirus del ZikaCoinfeccionesColombiaDengue virusZika VirusCoinfectionColombia