Jarma Orozco, Alfredo de JesúsCastillejo Morales, Ángela María2020-07-152020-07-152020-06-09https://repositorio.unicordoba.edu.co/handle/ucordoba/3378The Stevia rebaudiana (Bertoni) species is an herbaceous plant, belonging to the genus Stevia Cav. characteristic of the American continent, member of the Eupatorieae tribe, family Asteraceae (Flores y Espinosa, 2018). It is a plant widely spread throughout the world since its leaves produce diterpene glycosides (steviosides and rebaudiosides), lowcalorie, non-toxic and high-potency sweeteners that can replace sucrose and other synthetic sweeteners, being 300 times sweeter than sucrose (A. Abbas Momtazi-Borojeni, S.-A. Esmaeili, E. Abdollahi, and AJC pd Sahebkar, 2017). The use of stevia has increased as a consequence of the awareness that the use of sucrose has raised about health. Beyond its value as a sweetener, stevia and its glycosides have therapeutic effects against various diseases such as cancer, diabetes mellitus, hypertension, inflammation, cystic fibrosis, obesity and dental caries. Studies have shown that the steviol glycosides found in stevia are not teratogenic, mutagenic, or carcinogenic and do not cause acute and subacute toxicity (A. Abbas Momtazi-Borojeni, S.-A. Esmaeili, E. Abdollahi, y A. Sahebkar, 2017). In addition to the benefits in human health, the cultivation of stevia implies the generation of jobs favoring the peasant and small producers labor force. In this sense, the University of Córdoba has developed adaptation and management studies in clones and promising stevia lines, which demonstrate the potential for planting in the Colombian Caribbean thanks to its agro-ecological diversity. However, the behavior of these promising new lines in the face of current and future changes, as a consequence of unsustainable anthropic activities such as the salinization of soils, is unknown. With the growth of the stevia market, the need to increase the production area has been generated, however, in the department of Córdoba, salinization problems arise, which affect crop yields, making it necessary To know the aspects applied to the productivity of stevia plants under saline stress conditions. Therefore, the purpose of this research work was to evaluate the response of growth parameters, gas exchange and photosynthetic pigments of Stevia rebaudiana Bert., To different concentrations of salts (0; 0.2; 0.5; 1, 2; 2.0 and 3.0 meq Na / 100g) in two genotypes (C19 and C04) under greenhouse conditions. Chapter II shows the study of the effect of five levels of salinization under greenhouse conditions on the production and distribution of stevia biomass. The relationship between growth and salt stress of the two stevia genotypes studied in the present investigation has a decreasing trend as NaCl doses are increased. The biomass located in the aerial organs is significantly affected with levels of 0.2 meq Na / 100g. Both genotypes showed a 8 tendency to increase in root biomass at the highest NaCl doses (2.0 and 3.0 meq Na / 100g), which could be considered a defense physiological response to osmotic stress. In Chapter III, the gas exchange response is shown in two genotypes of Stevia rebaudiana Bert. under five concentrations of salts. The most relevant results showed a differential behavior in the variables of gas exchange, DPV, internal leaf temperature and Ci / Ca, which could be explained by the effect of gene expression. While stomatal conductance and perspiration values did not report effects of NaCl doses and / or genotypes. The reduction in the rate of CO2 assimilation is associated with increased salinity but is not related to stomatal effects, suggesting an oxidative response of plants to saline stress conditions.1. CAPÍTULO I. INTRODUCCIÓN GENERAL .................................................................. 161.1 INTRODUCCIÓN.......................................................................... 161.2 PLANTEAMIENTO DEL PROBLEMA ..................................... 171.3. STEVIA (Stevia rebaudiana Bert) ......................................... 191.3.1. Generalidades de la planta de Stevia rebaudiana Bert ......................................... 191.3.2 Clasificación sistemática ...................................................... 201.3.2 Efecto del estrés salino en las plantas ............................... 211.3.3 Mecanismos bioquímicos y moleculares de tolerancia a la sal ........................ 221.3.4 Comportamiento de la fotosíntesis bajo condiciones de estrés salino. .................. 231.4 OBJETIVOS................................................................................ 241.4.1. Objetivo General ................................................................. 241.4.2 Objetivos específicos ...................................................... 241.5 LITERATURA CITADA ................................................................ 252. CAPITULO II. DISTRIBUCIÓN ESPACIAL DE LA BIOMASA EN DOS GENOTIPOS DE Stevia rebaudiana Bert. BAJO CINCO NIVELES DE SALINIDAD ..................................... 272.1. RESUMEN ....................................................................... 272.2. ABSTRACT......................................................................... 272.3. INTRODUCCIÓN............................................................... 282.4. MATERIALES Y MÉTODOS .................................................... 312.4.1. Localización y material experimental ..................................................................... 312.4.1.1. Localización .......................................................................... 312.4.1.2. Variables e indicadores ....................................................... 312.4.1.2.1. Variables independientes. ............................................... 312.4.1.2.2. Variables dependientes. ......................................... 312.4.1.3. Procedimiento ............................................. 322.4.1.3.1. Preparación de semillero ................................................ 322.4.1.3.2. Montaje del ensayo .......................................... 332.4.1.3.3. Preparación de soluciones salinas ............................................................................ 332.4.1.3.4. Obtención de esquejes ................................................... 342.4.1.3.5. Trasplante ...................................................... 342.4.1.3.6. Toma de datos. ................................................................. 352.4.1.3.7. Diseño Experimental.................................................. 352.4.2. Análisis de la información ....................................................................................... 352.5. RESULTADOS Y DISCUSIÓN ..................................................................................... 362.5.1. PRODUCCCION DE BIOMASA. ............................................................................ 362.5.1.1. Biomasa de hoja ................................................................... 362.5.1.2. Biomasa de tallo .................................................................. 382.5.1.3. Biomasa de raíz ............................................................. 402.5.1.4. Biomasa total .................................................................. 422.5.1.5. Relación raíz – dosel (RRD) ......................................... 452.5.1.6. Índice de Cosecha (IC) ...................................................... 462.6. CONCLUSIONES ................................................................. 472.7 LITERATURA CITADA ................................................................ 493. CAPITULO III. INTERCAMBIO GASEOSO EN DOS GENOTIPOS DE Stevia rebaudiana Bert. BAJO CINCO CONCENTRACIONES DE SALES .................................. 543.1 RESUMEN ............................................................................. 543.2 ABSTRACT............................................................... 543.3 INTRODUCCIÓN ................................................................ 553.4 MATERIALES Y MÉTODOS ................................................... 573.4.1. Localización y material experimental. .................................................................... 573.4.1.1. Localización .................................................. 573.4.1.2. Variables e indicadores .................................................. 573.4.1.2.1. Variables independientes. ............................................... 573.4.1.2.2. Variables dependientes. ................................................ 573.4.1.3 Procedimiento .................................................................. 583.4.1.3.1. Preparación de semillero ................................................ 583.4.1.3.2. Montaje del ensayo ............................................ 583.4.1.3.3. Preparación de soluciones salinas ............................................................................ 583.4.1.3.4. Obtención de esquejes .............................................. 593.4.1.3.5. Trasplante ...................................................................... 593.4.1.3.6. Toma de datos. ........................................... 593.4.2. Análisis de la información ................................... 603.5. RESULTADOS Y DISCUSIÓN ........................................ 603.5.1. VARIABLES DE INTERCAMBIO GASEOSO DE Stevia rebaudiana Bert............ 603.5.1.1. Tasa de asimilación de CO2 (Pn) y pigmentos fotosintéticos. ............................ 613.5.1.2 Conductancia estomática y transpiración ............................................................ 653.5.1.3. Déficit de presión de vapor (DPV) y temperatura interna de la hoja (T° hoja) ... 673.5.1.4. Relación de la concentración de CO2 en el espacio subestomático (Ci/Ca) ..... 693.6 Conclusiones .......................................................... 703.7 LITERATURA CITADA ................................................................. 724. ANEXOS .................................................................... 77Anexo 1. Efecto de los tratamientos 15 días después del trasplante (DDT) ................... 77application/pdfspaCopyright Universidad de Córdoba, 2020Trabajo de grado - Pregradoinfo:eu-repo/semantics/closedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)SalinidadStevia rebaudiana (Bert)Distribución de biomasaIntercambio gaseosoPigmentos fotosintéticosConductancia estomáticaSalinityStevia rebaudiana (Bert)Biomass distributionGas exchangePhotosynthetic pigmentsStomatal conductance