Araméndiz Tatis, HermesQuiñónez Arrieta, Leidys LorenaSilva Vega, Yulissa Fernanda2020-11-032020-11-032020-11-03https://repositorio.unicordoba.edu.co/handle/ucordoba/3488Cowpea bean is a crop of social, economic and nutritional importance of the Colombian Caribbean, which according to some reports, has been affected in its yield, due to environmental changes especially high temperatures, which are increasing with the passing of the times. These fluctuations can cause negative effects on cowpea bean crop pollen viability. This research was carried out at Universidad de Córdoba aiming to evaluate the viability of pollen from three cowpea bean cultivars collected at different hours (6.00, 8: 00, 10: 00 and 12:00) and to determine the effect of different environments (fridge and cold-room, 1,1 ±0,5°C y RH 68% y 5,6 ± 1,0°C y RH 48%),) on pollen viability storaged for 6 and 12 days, which was determined by using staining methods with 1% Acetocarmín and 3% tetrazolium. Pollen was obtained from the collection of flower buds in anthesis in which temperature and relative humidity records were taken at the time of each sampling, using a using a completely randomized experimental design with a factorial arrangement of three factors; 2 (storage conditions), 3 (conservation times) 3 (genotypes), with four repetitions for pollen conservation. Regarding the time of collection, it was used a completely randomized experimental design with a factorial arrangement of two factors; 4 (hours of collection) and 3 (genotypes) for a total of 12 treatments, with four repetitions. The results stated that storage conditions do not impact pollen viability. As a matter of fact, conservation time and each genotype pollen features do influence its viability. With acetocarmin test were obtained the highest percentage of viable pollen (90%), in contrast with tetrazolium test, which was 70% where the pollen collection time did not affect its viabilityINTRODUCCIÓN ................................................................................................... 151. OBJETIVOS ....................................................................................................... 181.1. OBJETIVO GENERAL .................................................................................... 181.2. OBJETIVOS ESPECÍFICOS ........................................................................... 182. HIPÓTESIS ........................................................................................................ 193. REVISIÓN DE LITERATURA ............................................................................. 203.1. GENERALIDADES DE LA ESPECIE............................................................... 203.2. ORIGEN E IMPORTANCIA ECONÓMICA DEL FRIJOL CAUPÍ ......................... 203.3. IMPORTANCIA SOCIAL ................................................................................. 213.4. FACTORES QUE INCIDEN EN LA FLORACIÓN DEL CULTIVO ....................... 223.5. FACTORES QUE AFECTAN LA VIABILIDAD DEL POLEN ................................... 233.6. BIOLOGÍA FLORAL ........................................................................................ 243.7. MORFOLOGÍA DE LOS GRANOS DE POLEN ............................................... 253.8. ALMACENAMIENTO DEL POLEN .................................................................. 263.9. ESTUDIOS RELACIONADOS CON LA VIABILIDAD DEL POLEN .................. 274. MATERIALES Y MÉTODOS .............................................................................. 294.1. LOCALIZACIÓN .............................................................................................. 294.2. MATERIALES ................................................................................................. 294.3. MÉTODOS ...................................................................................................... 304.3.1. Viabilidad del polen en función de la hora de colecta .................................. 304.3.2. Efecto del almacenamiento en la viabilidad del polen .................................. 335. RESULTADOS Y DISCUSIÓN .......................................................................... 375.1. VIABILIDAD DEL POLEN EN FUNCIÓN DE LA HORA DE COLECTA ........... 375.2. EFECTO DEL TIEMPO DE ALMACENAMIENTO EN LA VIABILIDAD DEL POLEN ................................................................................................................... 446. CONCLUSIONES .............................................................................................. 56REFERENCIAS ..................................................................................................... 57application/pdfspaCopyright Universidad de Córdoba, 2020Trabajo de grado - Pregradoinfo:eu-repo/semantics/restrictedAccessAtribución-NoComercial 4.0 Internacional (CC BY-NC 4.0)Frijol caupíPrueba de acetocarmínPrueba de tetrazolioCowpea beanAcetocarmin testTetrazolium test