Santafe Patiño, GilmarQuirós Rodríguez, Jorge AlexanderGuerra Salcedo, Ester Acsami2020-11-172020-11-172020-11-14https://repositorio.unicordoba.edu.co/handle/ucordoba/3642The fireworm Hermodice carunculata PALLAS, 1766 belongs to the family amphinomidae, is characterized by its hive bite and the presence of calcified quetas that cover its entire body, however, its aposematic coloration is the most striking of this species. These organisms have varied and bright colors and some can reach large size. With this work, antioxidant activity was evaluated by the methods of the radical DPPH• and ABTS+• of the primary methabolic extract of the species Hermodice carunculata of exposed environment and protected from the swell, collected in dry and rainy season, by means of free search and manually. Antioxidant activity was determined to have no significant results (IC50 > 1000 mg/ml), but IC5O values allowed the study to be brought to comparison of extracts by the possibility of active secondary metabolites. The mean inhibitory concentration (IC50) had variations between the two sectors, so that those in exposed environments were considered "better activity" as they had lower concentration than the rest. On the other hand, these values were influenced by the action of the swell, the values furthest from the activity, were associated with the low impact environments of the waves, therefore, the effect of temperature and precipitation was added to it, also the coloration and size was a fundamental factor to attribute it to the antioxidant activity of Hermodice carunculata. It was proven through an analysis of main components that yellow and large specimens had low activity compared to those of brown and smaller size.RESUMENABSTRACT1. INTRODUCCIÓN .........................................................................................12. OBJETIVOS .................................................................................................32.1. General.....................................................................................................32.2. Específicos ...............................................................................................33. ESTADO DEL ARTE....................................................................................43.1 Antecedentes.........................................................................................43.2. Marco Teórico..............................................................................................63.2.1. Hermodice carunculata..........................................................................63.2.2. Estrés oxidativo .....................................................................................73.2.3. Actividad antioxidante...........................................................................73.2.4. Compuestos bioactivos........................................................................73.2.5 Aposematismo........................................................................................83.2.6. Ambientes protegidos del oleaje............................................................83.2.7. Ambientes expuestos al oleaje .............................................................84. DISEÑO METODOLÓGICO.........................................................................94.1. Área de estudio........................................................................................94.2 Fase de Campo .....................................................................................104.2.1. Recolección del material biológico.....................................................104.2.2. Medición de las variables ambientales ................................................114.3 Fase de laboratorio................................................................................114.3.1 Obtención del extracto........................................................................114.3.2 Determinación de la actividad antioxidante ..........................................114.3.2.1 Método radical DPPH ......................................................................124.3.2.1.2 Preparación solución madre de DPPH: ........................................124.3.2.1.3 Preparación de la solución madre del extracto...............................124.3.2.1.4 Evaluación de la muestra DPPH.....................................................124.3.2.2. Método radical ABTS........................................................................134.3.2.2.1 Preparación solución madre de ABTS:.........................................134.3.2.2.2 Preparación de la solución madre del extracto .............................134.3.2.2.3 Evaluación de la muestra ABTS. ....................................................144.4. Análisis de datos.....................................................................................155. RESULTADOS...........................................................................................165.1 Ensayo de decoloración del radical 1-1-Difenil-2-Picrilhidrazilo (DPPH•) y 2,2-azinobis-(ácido3-etilbenzotialzolina–6-sulfónico) (ABTS•+ ). ....................165.1.1 Preparación de la curva de referencia ..................................................165.1.1.2 Concentración inhibitoria media (IC50)..............................................185.1.1.2.1 Determinación del IC50 para el radical DPPH•...............................185.1.1.2.2 Determinación del IC50 para el radical ABTS+ •..............................225.2 Variables de estudio ................................................................................275.2.1 Análisis por sector ................................................................................285.2.2 Análisis por época ................................................................................295.3. Análisis de componentes principales (ACP) ...........................................306. DISCUSIÓN ...............................................................................................326.1 Actividad antioxidante..........................................................................326.2 Actividad antioxidante de ambiente protegido y expuesto al oleaje.........336.3 Actividad antioxidante de Hermodice carunculata asociada a talla y coloración. .....................................................................................................357. CONCLUSIONES.......................................................................................378. RECOMENDACIONES ..............................................................................389. BIBLIOGRAFIA ..........................................................................................3910. ANEXOS.....................................................................................................50application/pdfspaCopyright Universidad de Córdoba, 2020Trabajo de grado - Pregradoinfo:eu-repo/semantics/restrictedAccessAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)Gusano de fuegoActividad antioxidanteOleajeColoraciónCompuestos activosMetabolitos secundarios.FirewormAntioxidant activitySwellColorationActive compoundsSecondary metabolites