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dc.contributor.advisorAramendiz Tatis, Hermesspa
dc.contributor.authorHernández Murillo, Jenry Rafael
dc.date.accessioned2021-01-27T00:12:55Z
dc.date.available2021-01-27T00:12:55Z
dc.date.issued2021-01-25
dc.identifier.uriHttps://repositorio.unicordoba.edu.co/handle/ucordoba/3974
dc.description.abstractLa presente investigación se llevó a cabo en la Universidad de Córdoba, Montería; El objetivo fue determinar la viabilidad del polen de frijol caupí colectado a diferentes horas del día en los semestres agrícolas A y B de 2019, la receptividad estigmática, la conservación de polen en bajo las condiciones de nevera y cuarto frio y evaluar dos métodos de hibridación en frijol caupí. La viabilidad del polen se realizó mediante pruebas con acetocarmin y sal de tetrazolium, la receptividad del estigma se determinó con peróxido de hidrogeno. Se evaluó el porcentaje de granos de polen viable e inviable, el tiempo de reacción del estigma al peróxido de hidrógeno y el porcentaje de cruzamientos viables. El diseño utilizado fue completamente al azar con arreglo factorial; para viabilidad del polen a diferentes horas (2 test, 3 genotipos y 4 horas de colecta) con tres repeticiones (plantas); para receptividad del estigma (3 genotipos y 4 horas en el día) con tres repeticiones (flores) para cada ambiente; para el almacenamiento de polen (2 ambientes, 3 tiempos de almacenado y 3 genotipos) con cuatro (4) repeticiones por tratamiento. El polen colectado a diferentes horas del día en los genotipos evaluados en los semestres agrícolas promedio A y B de 2019, el cultivar que presentó mayor porcentaje de polen viable fue Caupicor 50 (93,89% y 94,61%). De acuerdo con el tiempo de reacción del estigma al peróxido de hidrógeno, las polinizaciones controladas que se realicen entre las 7:00 y 9:00 am en campo y entre las 3:00pm y 5:00pm en casa malla tendrán mayor probabilidad de generar vainas y semillas. El método de hibridación, emasculando y polinizando en la mañana de 7:00 a 10:00 am, en los dos ambientes se registraron los mayores porcentajes de cruzamientos viables. Las condiciones de temperatura en cuarto frieron (T = 1,1 ± 0,5°C) y nevera (T = 5,6 ± 1,0°C), permiten mantener por 12 días la viabilidad del polen de frijol caupí con promedios superiores al 70%, considerados óptimos para la polinización artificial.spa
dc.description.tableofcontentsRESUMEN GENERAL..........................................................................................................................xvispa
dc.description.tableofcontentsGENERAL ABSTRACT...........................................................................................................................xviispa
dc.description.tableofcontentsCAPITULO I: MARCO GENERALspa
dc.description.tableofcontents1. INTRODUCCIÓN GENERAL........................................................................................................20spa
dc.description.tableofcontents2. DEFINICIÓN DEL PROBLEMA......................................................................................................24spa
dc.description.tableofcontents3. GENERALIDADES DE LA TEMÁTICA................................................................................... 27spa
dc.description.tableofcontents3.1.1. Origen y Clasificación................................................................................................................27spa
dc.description.tableofcontents3.1.2. Requerimientos de la planta ............................................................................................28spa
dc.description.tableofcontents3.2. BIOLOGÍA FLORAL DEL FRIJOL CAUPI.........................................................................29spa
dc.description.tableofcontents3.3. VIABILIDAD DE POLEN............................................................................................................31spa
dc.description.tableofcontents3.4. RECEPTIVIDAD ESTIGMATICA...........................................................................................33spa
dc.description.tableofcontents3.5. CONSERVACIÓN DE POLEN..................................................................................................36spa
dc.description.tableofcontents3.6. MÉTODOS DE HIBRIDACIÓN EN FRIJOL CAUPI.....................................................38spa
dc.description.tableofcontents3.6.1. Emasculación...............................................................................................................................40spa
dc.description.tableofcontents3.6.2. Preparación de la flor masculina .....................................................................................41spa
dc.description.tableofcontents3.6.3. Hibridación artificial................................................................................................................42spa
dc.description.tableofcontents3.6.4. Identificación de los cruces................................................................................................43spa
dc.description.tableofcontents3.7. EFECTOS DEL AMBIENTE EN LA POLINIZACIÓN .................................................43spa
dc.description.tableofcontents4. OBJETIVOS..........................................................................................................................................50spa
dc.description.tableofcontents4.1. OBJETIVO GENERAL..................................................................................................................50spa
dc.description.tableofcontents4.2. OBJETIVOS ESPECÍFICOS.......................................................................................................50spa
dc.description.tableofcontents5. HIPÓTESIS ..............................................................................................................................................51spa
dc.description.tableofcontents6. PERFIL Y ALCANCE ......................................................................................................................52spa
dc.description.tableofcontents7. BIBLIOGRAFÍA ...................................................................................................................................54spa
dc.description.tableofcontentsCAPITULO II: VIABILIDAD DEL POLEN DE CULTIVARES DE FRIJOL CAUPI (Vigna unguiculata L. (Walp.)) COLECTADO EN DOS ÉPOCAS Y DIFERENTES HORAS DEL DÍA EN MONTERÍA - CÓRDOBA.spa
dc.description.tableofcontentsRESUMEN .................................................................................................................................................79spa
dc.description.tableofcontentsABSTRACT ..................................................................................................................................................80spa
dc.description.tableofcontents1. INTRODUCCIÓN ...............................................................................................................................81spa
dc.description.tableofcontents2. METODOLOGÍA EXPERIMENTAL ............................................................................................83spa
dc.description.tableofcontents2.1. LOCALIZACIÓN ................................................................................................................................83spa
dc.description.tableofcontents2.2. VARIABLES E INDICADORES ...............................................................................................83spa
dc.description.tableofcontents2.2.1. Variables dependientes .........................................................................................................83spa
dc.description.tableofcontents2.2.2. Variables independientes ....................................................................................................83spa
dc.description.tableofcontents2.3. PROCEDIMIENTO .......................................................................................................................83spa
dc.description.tableofcontents2.3.1. Viabilidad con acetocarmín ................................................................................................84spa
dc.description.tableofcontents2.3.2. Viabilidad con sal de tetrazolium ......................................................................................84spa
dc.description.tableofcontents2.4. ANÁLISIS ESTADÍSTICO ...........................................................................................................85spa
dc.description.tableofcontents3. RESULTADOS Y DISCUSIÓN .....................................................................................................87spa
dc.description.tableofcontents4. CONCLUSIONES ............................................................................................................................110spa
dc.description.tableofcontents5. BIBLIOGRAFÍA ....................................................................................................................................111spa
dc.description.tableofcontentsCAPITULO III: RECEPTIVIDAD ESTIGMÁTICA EN FRIJOL CAUPI (Vigna unguiculata L. (Walp.)) EN DIFERENTES HORAS DEL DÍA EN MONTERÍA – CÓRDOBA.spa
dc.description.tableofcontentsRESUMEN ...................................................................................................................................................124spa
dc.description.tableofcontentsABSTRACT .................................................................................................................................................125spa
dc.description.tableofcontents1. INTRODUCCIÓN ................................................................................................................................126spa
dc.description.tableofcontents2. METODOLOGÍA EXPERIMENTAL ..........................................................................................128spa
dc.description.tableofcontents2.1. LOCALIZACIÓN ...............................................................................................................................128spa
dc.description.tableofcontents2.2. VARIABLES E INDICADORES . ............................................................................................128spa
dc.description.tableofcontents2.2.1. Variables dependientes .......................................................................................................128spa
dc.description.tableofcontents2.2.2. Variables independientes ....................................................................................................128spa
dc.description.tableofcontents2.3. PROCEDIMIENTO ......................................................................................................................128spa
dc.description.tableofcontents2.4. ANÁLISIS ESTADÍSTICO .............................................................................................................129spa
dc.description.tableofcontents3. RESULTADOS Y DISCUSIÓN .....................................................................................................130spa
dc.description.tableofcontents4. CONCLUSIONES .................................................................................................................................136spa
dc.description.tableofcontents5. BIBLIOGRAFÍA ...................................................................................................................................137spa
dc.description.tableofcontentsCAPITULO IV: EVALUACIÓN DE DIFERENTES MÉTODOS DE HIBRIDACIÓN EN FRIJOL CAUPI (Vigna unguiculata L. (Walp.)) EN CONDICIONES DE CAMPO Y CASA MALLA EN MONTERÍA – CÓRDOBA.spa
dc.description.tableofcontentsRESUMEN .....................................................................................................................................................143spa
dc.description.tableofcontentsABSTRACT ....................................................................................................................................................144spa
dc.description.tableofcontents1. INTRODUCCIÓN .................................................................................................................................145spa
dc.description.tableofcontents2. METODOLOGÍA EXPERIMENTAL ...........................................................................................148spa
dc.description.tableofcontents2.1. LOCALIZACIÓN ....................................................................................................................................148spa
dc.description.tableofcontents2.2. VARIABLES E INDICADORES .................................................................................................148spa
dc.description.tableofcontents2.2.1. Variables dependientes ........................................................................................................148spa
dc.description.tableofcontents2.2.2. Variables independientes ..................................................................................................148spa
dc.description.tableofcontents2.3. PROCEDIMIENTO ...........................................................................................................................148spa
dc.description.tableofcontents2.4. ANÁLISIS ESTADÍSTICO .............................................................................................................150spa
dc.description.tableofcontents3. RESULTADOS Y DISCUSIÓN .....................................................................................................151spa
dc.description.tableofcontents4. CONCLUSIONES .............................................................................................................................159spa
dc.description.tableofcontents5. BIBLIOGRAFÍA ...................................................................................................................................160spa
dc.description.tableofcontentsCAPITULO V: EFECTO DEL ALMACENAMIENTO DE POLEN DE FRIJOL CAUPI (Vigna unguiculata L. (Walp.)) SOBRE LA VIABILIDAD EN DOS AMBIENTES EN MONTERÍA – CÓRDOBAspa
dc.description.tableofcontentsRESUMEN ...............................................................................................................................................167spa
dc.description.tableofcontentsABSTRACT ..................................................................................................................................................168spa
dc.description.tableofcontents1. INTRODUCCIÓN ................................................................................................................................169spa
dc.description.tableofcontents2. METODOLOGÍA EXPERIMENTAL ..........................................................................................171spa
dc.description.tableofcontents2.1. LOCALIZACIÓN .................................................................................................................................171spa
dc.description.tableofcontents2.2. VARIABLES E INDICADORES ................................................................................................171spa
dc.description.tableofcontents2.2.1. Variables dependientes ..........................................................................................................171spa
dc.description.tableofcontents2.2.2. Variables independientes ..................................................................................................171spa
dc.description.tableofcontents2.3. PROCEDIMIENTO ........................................................................................................................171spa
dc.description.tableofcontents2.4. ANÁLISIS ESTADÍSTICO .........................................................................................................172spa
dc.description.tableofcontents3. RESULTADOS Y DISCUSIÓN .....................................................................................................173spa
dc.description.tableofcontents4. CONCLUSIONES ...............................................................................................................................184spa
dc.description.tableofcontents5. BIBLIOGRAFÍA ......................................................................................................................................185spa
dc.description.tableofcontents6. CONCLUSIONES GENERALES ..................................................................................................191spa
dc.format.mimetypeApplication/pdfspa
dc.language.isoSpaspa
dc.rightsCopyright Universidad de Córdoba, 2021spa
dc.rights.uriHttps://creativecommons.org/licenses/by-nc-nd/4.0/spa
dc.titleEvaluación de la viabilidad del polen en cultivares de frijol caupí (Vigna unguiculata L. Walp.) en Montería-Córdobaspa
dc.typeTrabajo de grado - Pregradospa
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dc.rights.creativecommonsAtribución-NoComercial-SinDerivadas 4.0 Internacional (CC BY-NC-ND 4.0)spa
dc.subject.proposalHibridaciónspa
dc.subject.proposalTest de viabilidadspa
dc.subject.proposalConservaciónspa
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dc.subject.keywordsHybridizationspa
dc.subject.keywordsViability testspa
dc.subject.keywordsConservationspa
dc.description.degreelevelMaestríaspa
dc.description.degreenameMagíster en Ciencias Agronómicasspa
dc.publisher.facultyFacultad de Ciencias Agrícolasspa
dc.publisher.placeMontería, Córdoba, Colombiaspa
dc.publisher.programMaestría en Ciencias Agronómicasspa
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